To improve the detection sensitivity of low abundance proteins in samples,fluorescence labeling might be a good solution method.In this manuscript,with 5-({2-[(iodoacetyl)amino]ethyl} amino)naphthalene-1-sulfonic acid(1,5-I-AEDANS)as the derivatizing reagent,the cooperative effects of insulin and BSA during the derivatization were studied.Through HPLC analysis,it was found that with BSA added,the detection sensitivity of the derivatized insulin could be improved.When the concentration ratio of BSA to insulin reached 200∶1,the peak area of insulin was increased by over 4-fold,which demonstrates that the existence of BSA had a positive effect on the derivatization of insulin.Furthermore,even when the ratio reached 1000∶1,the S/N of insulin was 8,which could not be detected without BSA being added.However,on the contrary,with insulin being added,the peak area of the derivatized BSA was decreased,demonstrating that insulin had a negative effect on that of BSA.All these results might be of great significance to the detection of low abundance proteins in proteome study.
