＜正＞A novel riboflavin analog, 10-2’-hydroxyethylflavin （HEF）, has strong fluorescence, and it can be used as e...
LI,Hui JIANG, Zhi-Qin PAN, Yang YU, Shu-Qin (Depatment of Chemistry, Tongji University, Shanghai 200092) (Department of Chemical Physics, University of Science and Technology of China, Hefei 230026)
Photoinduced electron transfer reactions of N,N,N’, N’- tetramethylbenzidine (TMB) with several electron poor olefins in acetonitrile solution have been studied by using laser flash photolysis and steady-state fluorescence quenching method. The triplet state of TMB at 480nm was found to undergo reductive quenching with the elec- tron acceptors of fumaronitrile, dimethyl fumarate, cinnamonitrile, crotononitrile and diethyl fumarate. The detec- tion of ion radical intermediates in the photolysis reactions confirms the proposed electron transfer mechanism, as expected from thermodynamics. The quenching rate constants of triplet TMB were determined at 510nm, where all the kq values approach or reach to diffusion controlled limit. In addition, fluorescence quenching rate constant T kq was also obtained by calculating with Stern-Volmer equation.
