[Objective]The aim was to research the function of AtGEF1 in Rac/Rop GTPses mediate auxin signal passway.[Method]Using the transgenic plants of AtGEF1 promotor fused with GUS reporter gene and the over-expression plants of Rac/Rop GEF1 under the control of 35S promoter as materials,which were constructed from our lab,the expression pattern of GEF1 was analyzed by GUS assay using histochemical staining,and the development of seedling roots of over-expression plant of GEF1 was observed.[Result]GEF1 expression was mainly detected in root meristem,root vascular tissue,lateral roots and root hair.Furthermore,the expression level of GEF1 was highly increased with the induction of NAA.Over-expression of GEF1 was observed to enhance lateral root formation.[Conclusion]GEF1 may be involved in the regulation of development of root and root hair,and it may have redundant function in the control of lateral root development.
