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Assembly of ω-7 fatty acid biosynthesis pathway in tobacco vegetable organs for high-quality biodiesel production: Biofuel oil is a stable, low carbon, renewable liquid fuel for direct fossil fuel replacement or blending appl...; GAO Chang-YongLI Run-ZhiGAO Xiu-QingHUANG Chun-GuoZHANG LiWANG Ji-PingJIA Xiao-YunYANG Zhi-RongWU Yong-MeiMAO Xue

Plant lipid metabolic engineering for sustainable production of biofuel oil and industrial-valued chemicals: Vegetable oil, largely stored in the form of triacylglycerol (TAG) as a major carbon and energy reserve, is th...; MAO XueXUE JinaiGAO ChangyongYANG ZhirongJIA XiaoyunZHANG LiWANG JipingWU YongmeiLI Runzhi; 关键词：PLANT LIPID BIOFUEL INDUSTRIAL ACID ACID

植物ω-7脂肪酸的系统代谢工程被引量：20: 2011年; ω-7脂肪酸(C16:1△9,C18:1△11,C20:1△13),特别是棕榈油酸(C16:1△9)具有重要的工业、营养和医药价值。这些珍稀脂肪酸大多在一些野生植物的种子中合成,不能商业化生产。对普通油料作物的油脂代谢途径进行遗传修饰,使其种子大量合成并积累ω-7脂肪酸,已成为生物技术和可再生资源研究的一个热点领域。基因操作的主要靶标包括:不同来源的△9脱氢酶的应用、提高底物(C16:0)的浓度、共表达质体型和内质网型?9脱氢酶以及代谢物流的优化等。该文在解析ω-7脂肪酸生物合成途径及其调控网络的基础上,重点论述了ω-7脂肪酸代谢工程的技术策略、研究进展和存在的问题,并进一步讨论了油脂物组学和转基因组学等组学技术在鉴定参与ω-7脂肪酸生物合成途径及其调控的特异基因和优化油脂代谢工程设计上的应用前景。; 吴永美毛雪王书建薛金爱贾小云王计平杨致荣李润植; 关键词：代谢工程

Identification of substrate specificity of diacylglycerol acyltransferase 1 (MucDGAT1) encoded by a cDNA isolated from developing cat's claw seed: Diacylglycerol acyltransferase(DGAT) catalyzes the acyl-CoA-dependent acylation of sn-1,2-diacylglycerol(DAG) ...; MAO XueWU Yong-MeiXUE Jin-AiGAO Chang-YongLI Run-Zhi

Silencing of soybean Acyl-ACP thioesterase B (GmFatB) increases palmitoleic acid accumulation in soybean oil: Acyl-acyl cartier protein (ACP) thioesterases are enzymes that terminate the intraplastidial fatty acid synthe...; XUE Jin-AiLI Run-ZhiMAO XueYANG Zhi-RongJIA Xiao-YunWU Yong-MeiGAO Xiu-QingHUANG Chun-GuoZHANG LiWANG Ji-Ping

酿酒酵母脂酰-△9脱氢酶亚细胞定位表达及其对烟草脂肪酸合成的影响被引量：10: 2013年; 以烟草Nicotiana tabacum L.为宿主植物,分别在细胞质内质网和质体内定位表达酿酒酵母Saccharomyees cerevisiae脂酰-CoA-Δ9脱氢酶(ScΔ9D),以期提高植物组织中棕榈油酸(16∶1Δ9)的积累量和分析该酶不同亚细胞定位表达对油脂代谢的影响。与野生型和空载体(对照)植物相比,转基因烟草植株叶片中单不饱和的棕榈油酸及顺式十八碳烯酸(18∶1Δ11)含量明显提高,而饱和的棕榈酸(16∶0)含量相应减少,多不饱和的亚油酸(18∶2Δ9,12)和亚麻酸(18∶3Δ9,12,15)含量亦降低。ScΔ9D质体定位表达烟叶中棕榈油酸及顺式十八碳烯酸含量分别是ScΔ9D细胞质内质网定位表达烟叶的2.7和1.9倍。这表明酵母脂酰-Δ9脱氢酶能在高等植物细胞中正确催化棕榈酸(16∶0)转化为棕榈油酸(16∶1Δ9),而且在质体内表达的效应显著高于在细胞质内质网上的效应。新建立了一种应用脂酰-CoA-Δ9脱氢酶代谢工程培育植物组织高水平合成积累棕榈油酸等ω-7脂肪酸的策略,有助于在生物量大的烟叶等营养器官中组装ω-7脂肪酸合成途径以生产优质生物燃油。; 薛金爱毛雪吴永美杨致荣贾小云张莉王计平岳爱琴孙希平李润植; 关键词：棕榈油酸酿酒酵母烟草

Metabolic pathway assembly for increasing renewable oil production in plant seeds and green tissues: Plant oils, one of the world's most important renewable oil resources, are widely consumed as food while a...; Xue MaoChangyong GaoJinai XueZhirong YangXiaoyun JiaLi ZhangJiping WangYongrnei WuAiqin YueRunzhi Li; 关键词：FATTY

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山西省自然科学基金(2010011038-1)