您的位置: 专家智库 > >

国家自然科学基金(30300026)

作品数:10 被引量:192H指数:8
相关作者:王泽港葛才林罗时石马飞万定珍更多>>
相关机构:扬州大学江苏省疾病预防控制中心更多>>
发文基金:国家自然科学基金更多>>
相关领域:农业科学环境科学与工程化学工程生物学更多>>

文献类型

  • 10篇期刊文章
  • 2篇会议论文

领域

  • 8篇农业科学
  • 5篇环境科学与工...
  • 2篇化学工程
  • 1篇生物学

主题

  • 8篇水稻
  • 5篇三氯苯
  • 4篇蛋白
  • 4篇三氯
  • 3篇蛋白质
  • 3篇幼苗
  • 3篇胁迫
  • 3篇氯苯
  • 3篇白质
  • 3篇1,2,4-...
  • 2篇属对
  • 2篇水稻幼苗
  • 2篇同功酶
  • 2篇重金
  • 2篇重金属
  • 2篇污染
  • 2篇磷酸化
  • 2篇PROTEO...
  • 2篇RICE
  • 1篇蛋白磷酸化

机构

  • 8篇扬州大学
  • 1篇江苏省疾病预...

作者

  • 8篇葛才林
  • 8篇王泽港
  • 7篇罗时石
  • 5篇马飞
  • 5篇万定珍
  • 3篇杨建昌
  • 2篇殷朝珍
  • 2篇刘冲
  • 2篇骆剑峰
  • 2篇丁艳
  • 2篇商奇
  • 1篇高红明
  • 1篇王余龙
  • 1篇朱红霞
  • 1篇马飞
  • 1篇络剑峰
  • 1篇杨亚春
  • 1篇龚峥
  • 1篇杨小勇
  • 1篇郦志文

传媒

  • 2篇核农学报
  • 2篇农业环境科学...
  • 2篇Rice s...
  • 1篇中国农业科学
  • 1篇中国水稻科学
  • 1篇作物学报
  • 1篇Journa...

年份

  • 2篇2009
  • 1篇2008
  • 3篇2007
  • 2篇2006
  • 3篇2005
  • 1篇2004
10 条 记 录,以下是 1-10
排序方式:
水稻根系对1,2,4-三氯苯胁迫的应答(英文)被引量:5
2007年
应用蛋白质组学、电镜观察、脂质过氧化物测定等方法研究了两个不同类型水稻品种的根系对有机污染物1,2,4-三氯苯(TCB)胁迫的应答。结果表明,TCB胁迫抑制水稻根系生长,影响根细胞结构,导致脂质过氧化。TCB胁迫对水稻根系蛋白质组也有显著影响。TCB胁迫能诱导根系内不同类型蛋白质的表达,它们是:解毒酶(包括脂酶、醛/酮还原酶、谷胱甘肽-硫转移酶),细胞壁物质代谢相关酶(包括蛋白转葡萄糖基酶、GDP-甘露糖-3,5-异构酶1),激素代谢或调节相关酶(包括酸式-还原酮二加氧酶、β-葡萄糖苷酶、病程相关蛋白家族10中的2个不同蛋白),原初或次生代谢相关酶(包括转录延长因子、细胞质磷酸丙酮酸双激酶、磷酸丙糖异构酶、丙氨酸氨基转移酶和异黄酮还原酶)。TCB胁迫诱导矮仔占根系中β-葡萄糖苷酶和病程相关蛋白家族10蛋白的表达,及汕优63根系中谷胱甘肽-S-转移酶和酸式-还原酮二加氧酶的表达,这种诱导蛋白的差异可能是汕优63较矮仔占对TCB胁迫有较高耐性的机理之一。
葛才林万定珍王泽港丁艳王余龙商奇马飞罗时石
关键词:水稻蛋白质组胁迫应答
Effects of 1,2,4-Trichlorobenzene and Mercury Ion Stress on Ca^2+ Fluxion and Protein Phosphorylation in Rice被引量:3
2007年
The effects of 5 mg/L 1,2,4-trichlorobenzene (TCB) and 0.1 mmol/L mercury ion (Hg2+) stresses on Ca2+ fluxion and protein phosphorylation in rice seedlings were investigated by isotope exchange kinetics and in vitro phosphorylation assay. The Ca2+ absorption in rice leaves and Ca2+ transportation from roots to leaves were promoted significantly in response to Hg2+ and TCB treatments for 4-48 h. The Ca2+ absorption peaks presented in the leaves when the rice seedlings were exposed to Hg2+ for 8-12 h or to TCB for 12-24 h. Several Ca2+ absorption peaks presented in the roots during rice seedlings being exposed to Hg2+ and TCB, and the first Ca2+ absorption peak was at 8 h after being exposed to Hg2+ and TCB. The result of isotope exchange kinetic analysis confirmed that short-term (8 h) Hg2+ and TCB stresses caused Ca2+ channels or pumps located on plasmalemma to open transiently. The phosphorylation assay showed that short-term TCB stress enhanced protein phosphorylation in rice roots (TCB treatment for 4-8 h) and leaves (TCB treatment for 4-24 h), and short-term (4-8 h) Hg2+ stress also enhanced protein phosphorylation in rice leaves. The enhancement of protein phosphorylation in both roots and leaves corresponded with the first Ca2+ absorption peak, which confirmed that the enhancement of protein phosphorylation caused by TCB or Hg2+ stress might be partly triggered by the increases of cytosolic calcium. TCB treatment over 12 h inhibited protein phosphorylation in rice roots, which might be partly due to that TCB stress suppressed the protein kinase activity. Whereas, Hg2+ treatment inhibited protein phosphorylation in rice roots, and Hg2+ treatment over 12 h inhibited protein phosphorylation in rice leaves. This might be attributed to that not only the protein kinase activity, but also the expressions of phosphorylation proteins were restrained by Hg2+ stress.
GE Cai-lin WANG Ze-gang WAN Ding-zhen DING Yan WANG Yu-long SHANG Qi LUO Shi-shi MA Fei
关键词:钙元素稻子
1,2,4-三氯苯和萘对水稻产量及品质的影响被引量:23
2006年
以汕优63和武运粳7号为材料,研究了两种有机污染物1,2,4-三氯苯(TCB)和萘(NAP)对水稻产量及其构成因子的影响并提出了减少籽粒中残留的调控措施。TCB明显降低两个水稻品种的移栽成活率,这是导致产量明显降低的主要原因;NAP对水稻移栽成活率影响较小。两种有机污染物都引起水稻抽穗期延迟。在产量构成因子中,每盆穗数和结实率是影响产量的两个主要因子。两种有机污染物均影响稻米品质,对稻米的垩白度影响最明显,其次是精米率、整精米率、蛋白质含量和直链淀粉含量,而对出糙率几乎无影响。TCB和NAP在水稻籽粒中都有残留,而NAP的含量几乎是TCB的10倍,表明NAP的生物可利用性要比TCB高。汕优63籽粒积累两种有机物的能力明显高于武运粳7号。增施有机肥以及N、P、K肥可明显减轻有机污染物的毒害作用,从而提高产量。上述结果说明,在污水灌溉实践中要注意控制秧苗移栽期灌溉水的质量,严格控制水中TCB尤其是NAP的含量,并通过适当的肥水等栽培措施进行调控,以保证产量和粮食安全。
王泽港万定珍杨亚春葛才林马飞杨建昌
关键词:1,2,4-三氯苯水稻有机污染物
Responses of wheat seedlings to cadmium,mercury and trichlorobenzene stresses被引量:12
2009年
The molecular response of wheat(Triticum aestivum L.,cv.Yangmai 13) seedlings to heavy metal(Cd,Hg) and 1,2,4-trichlorobenzene(TCB) stresses were examined by two-dimensional gel electrophoresis,image analysis,and peptide mass fingerprinting.The results showed inhibitions of root and shoot growth by Cd,Hg,and TCB.These stresses led to water deficit and lipid phosphorylation in the seedling which also promoted protein phophorylation in the leaves.Hg stress inhibited protein synthesis while Cd and TCB stresses induced or up-regulated more proteins in the leaves.Most of these induced proteins played important roles in the biochemical reactions involved in tolerance of wheat to Cd and TCB stresses.The primary functions of Cd-and TCB-induced proteins included methionine metabolism,Rubisco modification,protein phosphorylation regulation,protein configuration protection,H+ transmembrane transportation and also the synthesis of ethylene,defense substances and cell wall compounds.
GE CailinDING YanWANG ZegangWAN DingzhenWANG YulongSHANG QiLUO Shishi
关键词:小麦幼苗三氯苯RUBISCO活化酶蛋白质合成蛋白磷酸化
Proteomic study for rice seedling responses to cadmium stress
<正>The effect of heavy metals Cd~(2+) on the proteome in rice(Oryza sativa L.) seedlings was investigated by t...
Cai-lin Ge~(**1),Ze-Gang Wang,Ding-Zhen Wan,Yan Ding, Yu-Long Wang,Qi Shang and Shi-Shi Luo (Jiangsu Provencial Key Laboratory Of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,China)
关键词:RICEPROTEOME
文献传递
重金属对水稻光合作用和同化物输配的影响被引量:53
2005年
光合速率测定表明,0.025~1.0mmolL的Cu2+、Cd2+和Hg2+显著抑制水稻叶片的光合速率,且抑制程度随Cu2+、Cd2+和Hg2+浓度的增高而增强。示踪动力学分析表明,0.5mmolL的Cu2+、Cd2+和Hg2+抑制光合产物在叶内不同状态间的转化,影响叶中光合产物的输出,导致叶内小分子糖的累积,使叶片光合速率受糖浓度增高的反馈调节而下降。电泳分析表明,Cu2+、Cd2+和Hg2+抑制叶内淀粉酶同功酶的表达,并进而抑制光合同化物在叶内可输配态和暂贮态之间的转化。
葛才林骆剑峰刘冲殷朝珍王泽港马飞罗时石
关键词:光合作用输配CU^2+HG^2+CD^2+叶片光合速率
重金属对水稻过氧化物酶同功酶的影响被引量:33
2004年
对重金属胁迫条件下扬稻 6号体内POD活性测定和POD同功酶的聚丙烯酰胺浓度梯度凝胶电泳分析表明 ,0 0 5mmol L的Cu2 +、Cd2 +和Hg2 +诱导扬稻 6号叶片和根系POD同功酶活性较对照提高 ,但≥ 0 5mmol L的Cu2 +、Cd2 +和Hg2 +抑制叶片分子量较小的POD同功酶的表达。 0 5~ 1 0mmol L的Cu2 +、Cd2 +和Hg2 +强烈抑制根系POD同功酶的表达。 0 0 5~1 0mmol L的Cu2 +、Cd2 +和Hg2 +显著诱导穗中POS同功酶的表达。
朱红霞杨小勇葛才林龚峥王泽港罗时石马飞
关键词:重金属水稻过氧化物酶同功酶影响因素
1,2,4-三氯苯和萘对水稻幼苗生长的影响被引量:29
2006年
以水稻汕优63和武运粳7号为材料,在室外遮雨塑料棚下进行培养与处理,研究了1,2,4-三氯苯(TCB)和萘(NAP)对水稻种子萌发、幼苗生长及生理生化的影响。结果表明:①TCB和NAP对水稻种子发芽率几乎无影响,对幼苗株高、根长、根数有显著抑制作用,在水培条件下,较低浓度的TCB(10mg.L-1)、NAP(20mg.L-1)可显著抑制根系的伸长生长,但低浓度的有机污染物促进武运粳7号根数增加;②低浓度的TCB和NAP对水稻幼苗根系活力影响较小,但高浓度的污染物则显著抑制根系活力,影响根的吸收功能;③两有机污染物均导致水稻幼苗叶绿素含量的下降,Rubisco酶活性也明显受到抑制;④叶片中ATP含量随处理浓度增加而呈先降低后升高的变化趋势,而根系中ATP含量则呈先升高后降低的趋势;⑤地上部和根系中丙二醛(MDA)含量均随有机污染物浓度的增加而呈上升的变化趋势。研究结果显示,水中微量的1,2,4-三氯苯和萘均会明显影响水稻幼苗的生长,这对水稻育秧期水质管理具有重要的参考意义,同时,根长可作为确定有机污染物发生事件的植物形态学参考指标。
王泽港葛才林万定珍郦志文罗时石杨建昌
关键词:水稻幼苗
Proteomic Study for Responses to Cadmium Stress in Rice Seedlings被引量:8
2009年
A proteomic approach including two-dimensional electrophoresis and mass spectrometric (MALDI-TOF MS) analyses was used to investigate the responses to cadmium (Cd) stress in seedlings of rice (Oryza sativa L.) varieties Shanyou 63 and Aizaizhan. Cd stress significantly inhibited root and shoot growth, and affected the global proteome in rice roots and leaves, which induced or upregulated the expression of corresponding proteins in rice roots and leaves when rice seedlings were exposed to 0.1 or 1.0 mmol/L Cd. The Cd-induced proteins are involved in chelation and compartmentation of Cd, elimination of active oxygen free radicals, detoxification of toxic substances, degradation of denatured proteins or inactivated enzymes, regulation of physiologic metabolism and induction of pathogenesis-related proteins. Comparing the Cd-induced proteins between the two varieties, the β-glucosidase and pathogenesis-related protein family 10 proteins were more drastically induced by Cd stress in roots and leaves of Aizaizhan, and the UDP-glucose protein transglucosylase and translational elongation factor Tu were induced by 0.1 mmol/L Cd stress in roots of Shanyou 63. This may be one of the important mechanisms for higher tolerance to Cd stress in Shanyou 63 than in Aizaizhan.
GE Cai-linWANG Ze-gangWAN Ding-zhenDING YanWANG Yu-longSHANG QiLUO Shi-shi
关键词:水稻幼苗镉胁迫病程相关蛋白葡萄糖苷酶诱导蛋白
重金属对水稻呼吸速率及相关同功酶影响的研究被引量:20
2005年
采用室内培养液培养的方法,研究了重金属对水稻呼吸速率及相关同功酶的影响。结果表明,较高浓度(≥0.1m m ol·L-1)的重金属C u2+、C d2+和H g2+显著抑制扬稻6号和武运粳8号叶片的呼吸速率;供试浓度(0.025~1.0m m ol·L-1)的Cu2+、C d2+和H g2+强烈抑制上述两水稻品种根系的呼吸速率。对呼吸作用相关的几个重要酶同功酶的电泳分析表明,C u2+、C d2+、H g2+可通过影响水稻叶片和根系中的淀粉酶、苹果酸脱氢酶及细胞色素氧化酶同功酶来表达对水稻呼吸作用产生的影响,其中高浓度(≥0.5m m ol·L-1)C u2+、C d2+、H g2+抑制水稻叶片和根系呼吸作用的最主要原因与细胞色素氧化酶同功酶表达受到C u2+、C d2+、H g2+的抑制相关。
葛才林络剑峰刘冲殷朝珍王泽港马飞罗时石
关键词:重金属水稻呼吸速率同功酶
共2页<12>
聚类工具0