Objective Hypoglycemic Ziyabiti Tablets(HZT)is a traditional multicomponent treatment for diabetes in Xinjiang Uyghur Traditional Medicine.The present study aimed to investigate the effect of HZT in diabetic rats and its activity in cultured liver cells to investigate the relative mechanisms.Methods 10 days high-fat diet fed rats were intraperitoneally injected with alloxan(ALX)at next two subsequent days to induce diabetes mellitus(DM).Then were divided into 5 groups:saline,positive DM control and DM groups treated with different doses of HZT.Fasting blood glucose(FBG),total cholesterol(TC),total triglycerides(TG),high-density lipoprotein(HDL-C),fasting insulin(FI),insulin secretion(IS)and insulin sensitivity index(ISI)were measured.The IC_(50) of HZT in L-02 cells was determined by MTT assay,in intact and in paracetamol-induced liver injury(Par),on lactate dehydrogenase(LDH)activity and on glucose consumption.Results HZT decreased FBG and TC(P <0.05),increased IS(P <0.05)and at 440 mg·kg^(-1)·d^(-1) increased FI(P < 0.01).In vitro,HZT at 0.1,0.2,and 0.4 mg/mL decreased LDH activity and promoted glucose consumption.Conclusion The hypoglycemic mechanism of HZT is possibly related to increased insulin secretion from the pancreas and increased utilization of glucose by the liver.
Objective The present study investigated the effects of pomegranate ethyl acetate extract(PEE)on cell viability of primary rat islet cells.Methods PEE was extracted by using 95% ethanol and a series of organic solvents.Primary isolated islet cells of rat were cultured in high glucose and/or high fat conditions with or without PEE.Cell viability was determined by MTT assay.Insulin secretion was assessed by using radioimmunoassay.Results Insulin levels were significantly higher in cells treated with high glucose+PEE,palmitic acid+PEE,and olein acid+PEE compared to the cells treated with high glucose,palmitic acid,or oleic acid alone,in which the insulin levels were markedly less than that of the normal control group.Conclusion PEE promotes islet cell growth and insulin secretion,which is suggested that PEE can protect islet cell function in both high glucose and high fatty acid environments.
