随着都市宠物业的发展,伴侣动物的化脓性疾病日趋增多。为探究其影响病原,本研究对一例犬子宫蓄脓的浓汁进行了病原学鉴定。利用细菌学检测方法对采集到的病料进行分离纯化、涂片染色、镜检和生化鉴定,然后采用16S r RNA基因序列分析鉴定,发现该分离株的形态特征、生化结果与粪肠球菌一致,分离菌株的16S r RNA也与参考的粪肠球菌菌株同源性高达99%。药敏试验检测发现,该菌株对四环素、青霉素G、米诺环素、红霉素、诺氟沙星、环丙沙星、万古霉素、左氟沙星、克林霉素、多粘菌素B、氯霉素、复方新诺明和克拉霉素表现为耐药,对苯唑西林和呋喃妥因敏感。这些研究结果可为治疗此类多重耐药性肠球菌感染提供参考。
With the development of the urban pet industry,suppurative diseases of companion animals are increasing day by day.To explore its associated pathogens,this study was conducted on etilolgical identification from a case of a canine uterus with pus.Bacteriological detection methods were used to isolate and purify the collected disease materials,such as smear staining,microscopic examination and biochemical identification.Then,16S rRNA molecular sequence analysis was used to identify the isolate.It was found that the morphological features of the isolated strain and the biochemical results were consistent with Enterococcus faecalis.The 16S rRNA of the isolate was also as high as 99% homologous with the reference stains.Drug susceptibility test showed that the isolate was resistant to tetracycline,penicillin G,minocycline,erythromycin,ciprofloxacin,norfloxacin,vancomycin,levofloxacin,clindamycin,polymyxin B,chloramphenicol,compound sulfamethoxazole,and clarithromycin,and only susceptible to oxacillin and nitrofurantoin.These results provided reference for the treatment of this type of multidrug-resistant enterococcal infection.
In order to explore the genetic evolution of Actinobacillus pleuropneumoniae(App) in different countries and clarify the relationships among different App in each region, the 16 S rRNA gene of App in the NCBI nucleotide database was analyzed and compared by the bioinformatics method. The phylogenetic tree was constructed after tailoring alignment. The results showed that a stable genetic phenomenon was indicated in the evolutionary process of App. The isolates derived from China were clustered and showed a high degree of conservation. They had a certain genetic relationship with the British and American strains, but had far relationship with the strains from Japan which was a neighboring country of China. The isolates from different countries in the Eurasian continent shared high homology. The isolates of the two regions originated from common ancestors.
Pig Actinobacillus pleuropneumoniae(App) could induce chronic respiratory tract infection in pigs, which causes major economic losses on pig industry. Bacterial biofilm(BBF) is bacterial community adsorbed on the surface of biomaterials or body cavity, to protect bacteria escape, and recurrent outbreaks of related infectious diseases and chronic infections resulting therefrom are called bacterial biofilm diseases. App BF belongs to polymers with spatial structure in vitro, and its formation is regulated by multiple genes. Among them, gene deletion of the key component TolC of multidrug efflux pumps and type I secretion systems causes that App BF adhesion weakens; gene deletion of catalytic core ClpP of Clp proteolytic complex induces the inhibition of BF formation; outer membrane lipoprotein VacJ of App promotes BF formation; gene deletion of active enzyme LuxS enhances the formation of App BF and decreases bacterial adhesion ability; gene deletion of Adh obviously declines bacterial accumulation, BF formation and adhesion to host cells. In this paper, BF formation or inhibition mechanism in App is elaborated from molecular level, which could provide reference basis for exploring the prevention of its biofilm diseases.
