Fifty six endophytic bacteria strains were isolated from the roots of wheat,and one strain named G-32 was screened with antagonism on pathogenic fungi of Gaeumannomyces graminis var.tritici.Preliminary characterization indicated that G-32 was belonged to Bacillus cereus.The inhibitory action to the fungi was assayed in lab by using the fermentable extracts of antagonistic endophytic bacteria.The results showed that mycelium of G.graminis var.tritici growed slowly and the biomass reduced 89% compared with the control 6 d after inoculation.The hyphae were deformative which broke into pieces.Colonization was studied with dual-resistant label which suggested that the endophytic bacteria strain could colonize in the root systems of wheat.The control efficacy with G-32 strain against the disease of the wheat take-all in pot tests was better than that of common used chemical fungicide Diniconazole.
从小麦根际土壤分离得到枯草芽孢杆菌(Bacillus subtilis)B2-47拮抗菌株,室内测定其带菌培养液对小麦全蚀病菌(Gaeumannomyces graminis var tritici)9826菌株和9812菌株的抑制效果分别为61%和56%,盆栽试验中,其防治效果为63%和64%,高于化学杀菌剂处理的56%和54%的效果.实验表明,该菌株可以造成病菌菌丝发生畸变和菌丝细胞壁瓦解.
