Total 69 random primers were screened out through RAPD(Random Amplifi ed Polymorphic DNA) markers to analyze the genetic bands of 32 Kentucky bluegrass cultivars.A total of 197 bands were amplifi ed from 46 primers of them,among which 195 bands were polymorphic.Each primer could amplify one to nine polymorphic bands with an average of 4.3 being amplifi ed from an average of primer.Based on the similarity coeff icient analysis of RAPD results,NTSYS software and cluster analysis by using average UPGMA method,the result showed that 18 of 32 cultivars were in group 1,three cultivars were in group 2,two cultivars were in group 3,eight cultivars were in group 4,and there was only one cultivar in group 5,which was C2.
A total of 69 random primers were screened by using random amplified polymorphic DNA (RAPD) markers to analyze the genetic bands of 32 Kentucky bluegrass cultivars. A total of 197 bands were amplified from 46 primers, among which 195 bands were polymorphic. Each primer could amplify one to nine polymorphic bands with an average of 4.3 per primer. Based on similarity coefficient analysis of RAPD results and by using NTSYS software to cluster analyze with the average UPGMA method, the result showed that 18 cultivars of the 32 were in group 1, three cultivars were in group 2, two cultivars were in group 3, eight cultivars were in group 4, and only one cultivar in group 5.
Wang ChaoLi XinGao Li-naZhang LuLiu WeiLiu Hui-minChen Ya-jun
