通过硅胶、大孔树脂、葡聚糖凝胶Sephadex LH-20和HPLC等多种色谱分离方法相结合,从长梗喉毛花Comas-toma pedunculatum(Royle ex D.Dou)Holub全草乙醇提取物的正丁醇萃取部位中分离得到13个化合物,其中9个皂苷类,4个黄酮碳苷类;根据化合物的理化性质和波谱数据鉴定化合物的结构为柴胡皂苷元F(1),3-O-β-D-呋糖基柴胡皂苷元F(2),风轮菜苷ⅩⅤ(3),柴胡皂苷A(4),6″-乙酰基柴胡皂苷A(5),风轮菜苷Ⅰ(6),柴胡苷Ⅰ(7),风轮菜苷Ⅻ(8),柴胡皂苷b3(9),异牡荆苷(10),当药黄素(11),异荭草素(12),3',4',5-三羟基-7-甲氧基-6-C-β-D-吡喃葡萄糖黄酮苷(13)。化合物1～10,12,13均为首次从该属植物中分离得到。
Objective Curcumin is extracted from the turmeric plant (Curcuma longa Linn.) and is widely used as a food additive and traditional medicine. The present study investigated the activity of curcumin against staurosporine (STS) toxicity in cell culture. Methods Rat hippocampal neurons in primary culture were exposed to STS (20 μmol/L) and treated with curcumin (20 μmol/L). Cell viability was tested by MTT assay and reactive oxygen species (ROS) were measured using the MitoSOX red mitochondrial superoxide indicator. Western blot was used to assess changes in the levels of caspase-3 (Csp3), heat shock protein 70 (Hsp70) and Akt. Results The results showed that curcumin protects against STS-induced cytotoxicity in rat hippocampal neurons. Csp3, Hsp70, Akt and ROS activation may be involved in this protection. Conclusion Curcumin could be a potential drug for combination with STS in cancer treatment to reduce the unwanted cytotoxicity of STS.
