Objective: To discuss the correlation among intracranial arterial stenosis and its risk factors. Methods: A total of 486 patients with transient ischemic attack(TIA) or ischemic cerebral infarction were examined using color doppler flow imaging(CDFI) and transcranial doppler ultrosonography(TCD). According to the degrees of extracranial arterial stenosis, patients with mild-to-moderate extracranial stenosis were classified into group A(435 cases) while those with constant severe stenosis or occlusion were classified into group B(51 cases). The differences between the two groups of risk factors were compared, and the multi-factor logistic regression analysis of risk factors associated with moderately severe intracranial arterial stenosis was performed. Results:(1) The risk factors that were significantly associated with intracranial arterial stenosis included age(P = 0.034) and gender(P = 0.044).(2) Intracranial artery stenosis was observed in both anterior and posterior cerebral arteries in patients with hypertension, diabetes, and coronary heart disease respectively(P < 0.05).(3) Compared with group A, patients in group B were older(P = 0.000), with a higher proportion of men(P = 0.037), and the intracranial arterial stenosis degrees were significantly higher(P = 0.013).(4) Multi-factor logistic regression analysis showed that diabetes is a risk factor for moderately severe intracranial arterial stenosis(P < 0.05), and hyperlipidemia is a protective factor(P = 0.012). Conclusions: Age, gender, hypertension, diabetes, coronary heart disease, and smoking are risk factors for the distribution of intracranial arterial stenosis. The degrees of intracranial arterial stenosis are related with extracranial arterial stenosis. Diabetes is a risk factor for moderately severe intracranial arterial stenosis while hyperlipidemia is a protective factor.
Objective: We aimed to provide an alternative cell source for cell therapy in leukoaraiosis(LA). Methods: Olfactory ensheathing cells(OECs) from the olfactory bulb were isolated,cultured, and purified. Next, the lentivirus carrying human VEGF165 gene was constructed and transfected into OECs. Results: The proliferative capacity of primary OECs was strong. OECs were infected with different multiplicity of infection. The expression level of VEGF was confirmed by real-time PCR with specific primers for GAPDH and VEGF, indicating that the genetically engineered OECs-VEGF produced VEGF with functional activity. Conclusions: Our data showed that these engineered OECs-VEGF highly express functional VEGF and retain the characteristics of astrocytes and Schwann cells,providing an alternative cell source for cell therapy in LA.
目的探讨人血清淀粉样蛋白A(SAA)与脑梗死急性期认知功能的关系。方法选择山东大学附属省立医院神经内科确诊的脑梗死急性期患者105例,采用简易精神状态检查(MMSE)评估患者认知功能。根据受教育年限不同,将患者分为受教育年数<6年组(n=36)和受教育年数≥6年组(n=69)。分析SAA与各个影响因素的相关性,包括年龄、性别、受教育程度、α2-巨球蛋白(α2-MG)、吸烟和其他脑血管疾病危险因素,以及与M M SE中各个认知领域的相关性。结果受教育年限<6年组患者M M SE评分以及时间和地点定向、短时记忆和图形执行力等认知域评分显著低于受教育年限≥6年组(P<0.05或P<0.01)。认知评估中,时间与地点定向评分在前循环梗死组(n=53)、后循环梗死组(n=44)和全循环梗死组(n=8)之间的分布差异有统计学意义(P<0.05)。Spearman相关性分析表明,SAA与α2-M G水平呈明显正相关(r=0.29,P<0.01);广义线性回归分析显示,SAA与时间和地点定向以及计算能力下降相关(P<0.05或P<0.01)。结论血清SAA水平升高与脑梗死急性期认知功能障碍有关,尤其是时间与地点定向和计算力障碍。
