松口蘑(Tricholoma matsutake)也称松茸,是具有重要经济和药用价值的野生食用菌,菌塘是其子实体发生发育的场所。本文采用土壤平板稀释技术研究了云南省6份松口蘑菌塘土壤可培养细菌,共获得了178条细菌的16S r DNA碱基序列,经分析分别属于4个门、18个属、38个OTUs,变形菌门(Proteobacteria)序列占58.43%,厚壁菌门(Firmicutes)占26.40%,拟杆菌门(Bacteroidetes)占10.67%,放线菌门(Actinobacteria)占4.49%,其中厚壁菌门的芽孢杆菌属(Bacillus)占24.72%,变形菌门的伯克氏菌属(Burkholderia)占21.34%,假单胞菌属(Pseudomonas)占11.24%,拟杆菌门的金黄杆菌属(Chryseobacterium)占10.67%。云南松口蘑菌塘土壤可培养细菌的多样性较为丰富。
Although the species Mattiwlomyces terfezioides(≡ Terfezia terfezioides) has been recorded from China several times but it is really rare taxon with important ecological and economic value,the conspecihcity with European material has never been tested by molecular data.We re-examined three specimens labelled as T.terfezioides,one as T.leonis and one as Terfezia sp.in the herbarium HMAS and obtained five ITS and three LSU sequences.Our morphological observation and DNA sequences show that one specimen(HMAS 83766) labelled as M.terfezioides turns out to be Choiromyces sp.and the other four are M.terfezioides.The ITS and(or) LSU sequences of the Chinese samples are identical with or with 99%similarity to those from the European samples,which fully confirms the presence of M.terfezioides in China.The species is currently known from northern China(Hebei Province,Beijing and Shanxi Province).This study shows that M terfezioides has a Euroasia distribution other than European endemism and such distribution might be explained by the co-occurrence with the potential host tree Robinia pseudoacacia.
