Based on the sequence data of the nuclear ribosomal DNA internal transcribed spacer(ITS) 1,5.8 S,and ITS 2,the molecular phylogeny was analyzed on Ulvaceae species collected from Qingdao coasts in summer of 2007,including 15 attached Ulva and Enteromorpha samples from 10 locations and 10 free-floating Enteromorpha samples from seven locations.The result supported the monophyly of all free-floating Enteromorpha samples,implying the unialgal composition of the free-floating Enteromorpha,and the attached Ulvaceae species from Qingdao coasts were grouped into other five clades,suggesting that they were not the biogeographic origin of the free-floating Enteromorpha in that season.
紫菜作为一种大型红藻,是目前世界上人工栽培经济价值最高的一种栽培海藻。全世界人工栽培海藻的总产值近3×10~9美元,其中紫菜的产值近2×10~9美元,约占2/3(费修绠等,1998)。紫菜不仅含有丰富的蛋白质、碳水化合物,而且各种维生素和矿物质的含量也特别丰富(Ruperez et al.,2002;Dinabandhu et al.,2002),其中紫菜的多糖及藻胆蛋白等成分具有药用价值,以紫菜为原料从中提取高附加值的产品。因此,利用紫菜可以开发保健食品和生物药物,其具有很大的市场价值和开发潜力。
The life cycle of seaweed Laminaria japonica involves a generation alternation between diploid sporophyte and haploid gametophte. The expression of foreign genes in sporophte has been proved. In this research, the recombinant expression in gametophyte was investigated by particle bombardment with the rt-PA gene encoding the recombinant human tissue-type plasminogen activator (Reteplase), which is a thrombolytic agent for acute myocardial infarction (AMI). Transgenic gametophytes were selected by their resistance to herbicide phosphiothricin (PPT), and proliferated in an established bubble column photo-bioreactor. According to the results from quantitative ELISA, Southern blotting, and fibrin agarose plate assay (FAPA) for bioactivity, it was showed that the rt-PA gene had been inte-grated into the genome of gametophytes of L. japonica, and the expression product showed the ex-pected bioactivity, implying the proper post-transcript modification in haploid gametophyte.
ZHANG YiChen1,2, JIANG Peng1, GAO JiangTao1,3, LIAO JianMin4, SUN ShiJing4, SHEN ZiLong4 & QIN Song1, 1 Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
