RNA silencing has been shown to function in the plant antivirus defense response,leading to viral RNA degradation induced by vsiRNA-containing RISC cleavage activity.Cucumber mosaic virus(CMV) 3′UTR sequences share a high conservation of nucleotide sequence and secondary structures that are important for CMV replication.Here,in an attempt to simultaneously target the multiple genomic and subgenomic RNAs of CMV for degradation,CMV 3′UTR were used to design hairpin RNA(hpRNA) to transform tobacco(Xanthi.nc) so as to constitutively produce viral siRNAs.Most of the transgenic plants expressing CMV Q strain(Q-CMV,subgroup Ⅱ strain) RNA3 3′UTR-derived hpRNA showed de-layed resistance to Q-CMV infection and exhibited recovery phenotypes.Compared with Q-CMV-in-oculated leaves,the upper leaves showed weak or no disease symptoms and a reduced accumulation level of viral RNAs.Together with transient assays,our results indicate that the 3′UTR-derived siRNAs were biologically active in targeting viral RNA for degradation.Recovery resistance in transgenic plants was also observed against subgroup IB strain SD-CMV infection,indicating a broad-spectrum anti-CMV effect of the 3′UTR-based antiviral silencing.Northern blot assays indicated that there was no strong correlation between the degree of resistance and the accumulation level of 3′UTR-derived siRNAs,suggesting that to target a highly structured RNA,such as the CMV 3′UTR,the quantity of siRNAs may not be the only determinant of silencing efficiency.Target RNA secondary structures may also affect target accessibility,siRNA-containing RISC-target recognition and the consequent antiviral effect.
