AIM: To investigate the chemical and bioactive constituents from the stems and leaves of Micromelum integerrimum. METHOD: The chemical constituents were isolated and purified by silica gel, Sephadex LH-20, and HPLC. Their structures were mainly elucidated on the basis of extensive 1D- and 2D-NMR spectroscopy and mass spectrometry. Their cytotoxicity and antimicrobial activities were tested by the SRB and turbidimetric methods, respectively. RESULTS: Two new phenylpropanoids and two known coumarins were obtained, and their structures were identified as microintegerrin A(1), microintegerrin B(2), scopoletin(3), and scopolin(4). All of the compounds were tested for their cytotoxicity against three cancer cell lines(HeLa, A549, and BGC-823) and for antimicrobial activity against the fungus Candida albicans and the bacterium Staphylococcus aureus. CONCLUSION: Two new phenylpropanoids 1 and 2 were isolated and identified from the stems and leaves of M. intgerrimum. None of the compounds showed cytotoxic or antimicrobial activity at the tested concentration of 20 ?g·mL–1.
WANG Zhi-YaoHE Wen-JunZHOU Wen-BingZENG Guang-ZhiYIN Zhi-QiZHAO Shou-XunTAN Ning-Hua
Two new cyclic nonapeptides,named clausenlanins A(1)and B(2),were isolated from the roots and rhizomes of Clausena lansium.Their structures were elucidated as cyclo-(Gly^(1)-L-Leu^(2)-L-Ile^(3)-L-Leu^(4)-L-Leu^(5)-L-Leu^(6)-L-Leu^(7)-L-Leu^(8)-L-Leu^(9))(1)and cyclo-(Gly^(1)-L-Leu^(2)-L-Val^(3)-L-Leu^(4)-L-Leu^(5)-L-Leu^(6)-L-Leu^(7)-L-Leu^(8)-L-Leu^(9))(2)respectively on the basis of extensive spectroscopic analysis,particularly 2D NMR spectra taken at the temperature of 338 or 303 K and MS.
