Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibroblasts.Methods Rat lung fibroblasts and alveolar macrophages were cultured.A transdifferentiation model of rat lung fibroblasts was established.Free silica was used as a stimulator for rat lung fibroblasts.Changes in α-SMA were detected by immunohistochemistry and Western blot,respectively.Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis(2-DE).Results Six protein spots were identified by mass spectrometry,including glyceraldehyde 3-phosphate-dehydrogenase,peroxiredoxin 5,heterogeneous nuclear ribonucleoprotein A2,transgelin 2,keratin K6 and vimentin.Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts.
Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts Methods Rat lung fibroblasts and rat alveolar macrophages were cultured.A transdifferentiation model of primary rat lung fibroblasts was induced by free silica.Levels ofα-SMA protein,IGF-IIR protein and mRNA were measured by immunocytochemistry,Western blot and RT-PCR,respectively.Lung fibroblasts were treated with Wortmannin.Results The expression levels ofα-SMA and IGF-IIR increased with the increasing free silica concentration and decreased after Wortmannin was used.Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.
