[ Object] The study aimed to discuss the decolorization on indigo dyeing wastewater by laccase from Coriolus versicolor. [ Method ] Firstly, the effects of temperature, pH, indigo concentration, HBT concentration, laccase dosage on the decolorization of indigo dyeing wastewater by laccase/HBT, and then the synergism of laccase and acid cellulase was analyzed. [Result] Using ABTS as the substrate, the kinetic parame- ters, K,, and Vmax, were 0.318 mmol/L and 0.035 5 mmol/（ L . min） respectively. The decolorization rate of indigo reached 96.5% when the lacca- se acted on indigo for 40 min with HBT as an introducer at temperature 50 ℃, pH =4.5, indigo concentration 100 mg/L, HBT concentration 0.1% and laccase dosage 100 lU/L. Due to the synergism of laccase and acid cellulase during the bio-finishing of blue jeans, the backstaining degree of blue jeans reduced by 85% when the amount of laccase added was 15 000 IU/kg. Menawhile, the synergism of the laccase and acid cellulase de- creased indigo concentration in wastewater by 83.8%. [ Conclusion ] The laccase from Coriolus versicolor had a good prospect in the bio-finishing of blue jeans and the decolorization of indigo dyeing wastewater.
Enli GaoWeijia ZhongXiaolan FuFushan ChenZhigang Ye
[Objective] To screen out a microbial flocculant with good decolorization effect on methyl orange wastewater,and study the effect of different cultural conditions on decolorization effect of methyl orange.[Method] Absorbance of methyl orange solution before and after decolorization was determined by spectrophotometer,and the decolorization rate was calculated to compare the effects of different cultural conditions on removal rate of methyl orange.[Result]An optimal actinomycete stain（F-1-2） was screened out,and the best cultural condition was as follows：with sucrose as carbon source and NaNO3 as nitrogen source,cultured in constant temperature oscillator at 150 r/min,30℃ for 72 h.Under the optimal condition,the removal rate against methyl orange could reach 68.4%.[Conclusion]Different culture conditions have great impact on decolorization effect of strain.
