Salt stress is one of the most serious abiotic stresses limiting plant growth and development.Calcium as an essential nutrient element and important signaling molecule plays an important role in ameliorating the adverse effect of salinity on plants.This study aimed to investigate the impact of exogenous calcium on improving salt tolerance in Tartary buckwheat cultivars,cv.Xinong9920(salt-tolerant)and cv.Xinong9909(salt-sensitive).Four-week-old Tartary buckwheat seedlings under 100 mM NaCl stress were treated with and without exogenous calcium chloride(CaCl_(2)),Ca^(2+)chelator ethylene glycol tetraacetic acid(EGTA)and Ca^(2+)-channel blocker lanthanum chloride(LaCl_(3))for 10 days.Then,some important physiological and biochemical indexes were determined.The results showed that salt stress significantly reduced seedling growth,decreased photosynthetic pigments,inhibited antioxidants and antioxidant enzyme activities.However,it increased the reactive oxygen species(ROS)levels in the two Tartary buckwheat cultivars.Exogenous 10 mM CaCl_(2)application on salt-stressed Tartary buckwheat seedlings obviously mitigated the negative effects of NaCl stress and partially restored seedlings growth.Ca^(2+)-treated salt-stressed seedlings diplayed a suppressed accumulation of ROS,increased the contents of total chlorophyll,soluble protein,proline and antioxidants,and elevated the activities of antioxidant enzymes compared with salt stress alone.On the contrary,the addition of 0.5 mM LaCl_(3)and 5 mM EGTA on salt-stressed Tartary buckwheat seedlings exhibited the opposite effects to those with CaCl_(2)treatment.These results indicate that exogenous Ca^(2+)can enhance salt stress tolerance and Ca^(2+)supplementation may be an effective practice to cultivate Tartary buckwheat in saline soils.
