目的通过基因芯片寻找大鼠激素性股骨头坏死（steriod—induced necrosis of femoralhead，SINFH）组织中表达变化的氧化应激相关基因，探究氧化应激诱发或加重SINFH的始动因素和分子机制。方法20只成年健康Wistar大鼠随机分为对照组和模型组，每组10只。模型组大鼠腹腔注射大肠杆菌内毒素后，于左侧臀肌注射大剂量甲基泼尼松龙，建立SINFI-I模型；正常对照组大鼠腹腔注射大肠杆菌内毒素后，于左侧臀肌注射相同剂量生理盐水。6周后取两组大鼠的左侧股骨头标本进行组织病理学观察，并抽提各组大鼠股骨头组织的mRNA，经反转录获得大鼠股骨头组织cDNA，进行基因芯片检测。检测结果经荧光定量PCR验证。结果组织病理学观察显示模型组大鼠股骨头出现骨小梁紊乱、变细，骨细胞坏死，空骨陷窝率增加；对照组大鼠未见股骨头坏死。模型组基因芯片检测发现27条氧化应激相关基因存在差异表达，其中COX6A2、COX412、SOD3和DUSPl等4条基因的表达与对照组存在显著差异，且均呈下调表达。这些显著差异表达基因的功能涉及抑制活性氧自由基（ROS）生成，加速ROS清除以及保护组织免受氧化损伤等方面。结论大鼠SINFH组织中氧化应激相关基因的表达存在变化，初步提示COX6A2、COX412、SOD5和DUSPl基因是氧化应激在SINFH发生、发展过程中起作用的关键基因。
Objective:To observe the regulation of Youguiyin(YGY,右归饮) on the gene expression profile of the rat with steroid-induced femoral head necrosis(sFHN),for the sake of investigating its molecular mechanism of sFHN prevention and treatment.Methods:All the 30 rats were randomly divided into three groups, the normal control group(A),the model control group(B),and the YGY treated group(C),10 in each group. After rats in Groups B and C were being made into FHN models with steroid injection,they received a daily intragastric administration of saline and YGY respectively in equal volume for a total of 6 weeks,while to the unmodeled normal rats in Group A,saline was administered instead.The rats were sacrificed at the terminal of administration;their mRNA from femoral head tissue was extracted and prepared to cDNA probe through inverse transcription for detecting gene expression profile by microarray,outcomes of which was passing fluorescence quantitative PCR verification,and the differential expressed genes were analyzed adopting gene ontology(GO) method.Results:Compared with Group A,the numbers of differential genes found in Groups B and C were 190 and 92,respectively,but the changing trend in the two groups was opposite,mainly manifested as down-regulating in Group B/Group A(GB/GA) and up-regulating in Group C/Group B(GC/GB).The analysis showed that these differential genes were mainly assigned to cell apoptosis,signal transduction,metabolism, cell proliferation and differentiation,cell cycle,blood coagulation,antioxidant activity,etc.Conclusions:sFHN was regulated by various genes;the regulation of YGY on expressions of these genes and the intra/extra-cellular signaling processes was possibly the molecular mechanism of YGY for preventing/treating sFHN.This study gave an explanation to the effectiveness of Chinese medicine in preventing/treating FHN from aspects of gene expression and enriched the Chinese medicine theory of "Kidney(Shen) governing bones".
