A chemical investigation on the cultures of Xylaria carpophila led to the isolation of one known cyclopeptide cyclo(N-methyl-L-Phe-L-Pro-L-Leu-D-Ile-L-Val)(1),five new sesquiterpenes,named as xylcarpins A-E(2-6),and another known compound(7).The structures were determined by extensive NMR and MS spectroscopic analysis.The absolute configuration of 1 was established by use of Marfey’s method and ROESY spectroscopic data.All compounds were tested for their cytotoxicities against five human cancer cell lines.Compound 7 showed week inhibitory activity.
Xia YINTao FENGZheng-Hui LIJia SUYan LINing-Hua TANJi-Kai LIU
Seven new illudin-type sesquiterpenoids,agrocybins A-G(1-7),along with three known analogues(8-10),have been isolated from the culture broth of the fungus Agrocybe salicacola.Their structures were elucidated on the basis of extensive spectroscopic data analysis and comparison with data reported in the literature.The relative stereoconfigurations of 1 and 6 were elucidated by the X-ray crystallographic diffraction analysis.Compound 1 was highly cyclized containing seven chiral carbons which arranged compactly in six rings.
Liang-Yan LIULing ZHANGTao FENGZheng-Hui LIZe-Jun DONGXing-Yao LIJia SUYan LIJi-Kai LIU
Investigation of the culture of basidiomycete Polyporus ellisii led to the isolation of a novel compound 3β,9α,15α-trihydroxy-(22E,24R)-10(5→4)-abeo-ergosta-6,8(14),22-trien-5-one(1)with a new 5/7/6/5 ring system of ergosterol skeleton.In addition,five new steroids,5β,6β-epoxy-3β,7α,9α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(2),5β,6β-epoxy-3β,7α-dihydroxy-(22E,24R)-ergosta-8(14),22-dien-15-one(3),5α,6α-epoxy-3β,9α,15α-trihydroxy-(22E,24R)-ergosta-8(14),22-dien-7-one(4),15α-acetoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(5),15β-methoxy-(22E,24R)-ergosta-4,6,8(14),22-tetraen-3-one(6),along with four known ergosterols(7-10),were obtained.All structures were elucidated based on 1D and 2D NMR spectral data.New compounds were evaluated for cytotoxicity against five human cancer cell lines,only compound 4 was found to exhibit a favorable cytotoxicity profile toward all tested tumor cell lines.
Shuang WANGLing ZHANGLiang-Yan LIUZe-Jun DONGZheng-Hui LIJi-Kai LIU
Three new sesquiterpene aryl esters,named 10-dehydroxy-melleoliede B(1),1-O-formyl-10-dehydroxy-melleoliede B(2)and 10-oxo-melleoliede B(3)together with six known ones(4-9),were isolated from the cultures of Armillaria sp.The structures of the new compounds were elucidated based on the extensive spectroscopic methods.Compounds 1,2,and 5-9 exhibited moderate cytotoxicities.
Seven cadinane sesquiterpenoids,named boreovibrins A-G(1-7),three dihydrobenzofurans(8-10),and two lactones(11 and 12),together with one known compound(13),were isolated from cultures of the basidiomycete Boreostereum vibrans.The new structures were elucidated by means of spectroscopic methods.Compounds 5,6,and 11 showed weak inhibitory activities against isozymes of 11β-hydroxysteroid dehydrogenases(11β-HSD).
Jian-Hai DINGTao FENGZheng-Hui LILiang LIJi-Kai LIU
Five new polyketides,craterellones A-E(1-5),were isolated from cultures of basidiomycete Craterellus odoratus,together with five known compounds(6-10).Structures of 1-5 were elucidated on the basis of extensive spectroscopic analysis.All compounds were evaluated for their inhibitory activities against one isozyme of 11β-hydroxysteroid dehydrogenase(11β-HSD1)and cytotoxic activities on five tumor cell lines.Compound 10 exhibited significant cytotoxicity against HL-60,SMMC-7721,A-549,MCF-7,and SW-480,with IC50 values of 0.50,0.69,0.64,1.10,0.54μM,respectively.
Three new benzene derivatives,named daldins A-C(1-3),together with a known analogue,2-hydroxymethyl-3-(1-hydroxypropyl)phenol(4)have been isolated from cultures of ascomycete Daldinia concentrica.The structures of 1-4 with absolute configuration were established by means of spectroscopic methods and X-ray diffraction.All compounds showed no significant inhibition on five human cancer cell lines with IC50 values>40μmol.
Tao FENGZheng-Hui LIXia YINZe-Jun DONGGang-Qiang WANGXing-Yao LIYan LIJi-Kai LIU
