An efficient and safe gene delivery system remains a challenge in the development of gene therapy.Polycation-based gene nanoparticles are a typical non-viral gene delivery system,which are able to transfect cells in vitro and in vivo.This paper reported a facile method for constructing biodegradable multilayers via layer-by-layer self-assembly,in which the polycation-based gene nanoparticles were loaded.Through this surface-mediated delivery system,adherent cells on the multilayer could be transfected in situ.Gene nanoparticles-loaded multilayers transfect cells with higher efficiency than naked DNA-loaded multilayers because of the complex configuration of the DNA.DNA nanoparticles/PGA multilayers constructed on the scaffold surface could also realize in situ transfection on the adherent cells.The well-structured,easy-processed multilayers may provide a novel approach to precisely controlled delivery of gene nanoparticles,which may have potential applications for gene therapy in tissue engineering and medical implants.
WANG XueFei,REN KeFeng,LIN QuanKui & JI Jian Key Laboratory of Macromolecule Synthesis and Functionalization,Ministry of Education
Thrombosis and in-stent restenosis constitute major clinical failures of endovascular stent implantation.In this research,a polyelectrolyte multilayered film of heparin/collagen was constructed onto the endovascular stent via layer-by-layer deposition.The in vitro human platelet adhesion test and blood clotting time assessments demonstrated that the fabricated multilayer took on excellent hemocompatibility.The in vitro human umbilical vein endothelial cell culture results show that the endothelial cell adhesion and proliferation are greatly promoted after the multilayer modification.Such multilayered endovascular stents with excellent hemocompatibilty and human endothelial cytocompatibility may have great potential in clinical applications.
