A modified method of preparing 20(S)-ginsenoside Rh_2(G-Rh_2) and the inhibitory effect of 20(S)-ginse-noside Rh_2 on Hep-A-22 cells were investigated.The total saponins and strong alkali were dissolved in glycerol at the atmospheric pressure,and the degradation was performed at a high temperature.After G-Rh_2 had been isolated and purified,MTT(methyl thiazolyl tetrazolium) assay was applied to evaluating the effect of 20(S)-ginsenoside Rh_2 on the cells viability and morphological changes were observed.It was shown that 20(S)-ginsenoside Rh_2 can reduce Hep-A-22 cells viability in dose-dependent manner and the cells took on cell shrinkage,membrane blebbing,chromosomal condensations,especially under the higher concentrations of it.In conclusion,20(S)-ginsenoside Rh_2 can be prepared effectively that not only decreases viability but also induces the apoptosis of Hep-A-22 cells.
Gross saponins of Tribulus terrestris(GSTT) have exact effect on cardiovascular and cerebrovascular diseases.But as a mixture,the specific efficient component of GSTT is still unknown.Nine monomers of spirostanol saponins were isolated and idendified as JA―JI(named transitorily) by means of NMR spectrometry.After bio-activity screening on them,we defined that monomers tigogenin 3-O-β-D-xylopyranosyl(1→2)-[β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-[α-L-rhamnopyranosyl(1→2)]-β-D-galactopyranoside(compound JB) and hecogenin3-O-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside(compound JG) have cytoprotective bio-activity.Compound JB display effective dose in 10-8 and 10-9 mol/L,and JG in 10-6―10-9 mol/L.Survival rate,lactate dehydrogenase(LDH) and apoptosis also show that JB(at dose 10-8,10-9 and 10-10 mol/L) can protect myocardial injury caused by hypoxia/reoxygenation(H/R).While morphology change also shows JG has cytoprotective bio-activity.
