The water-soluble polysaccharides isolated from the leaves of Panax ginseng C.A.Meyer were totally fractionated into one neutral fraction(GLPN-1),six acidic fractions(GLPN-2,GLPA-1a,GLPA-1b,GLPA-1c,GLPA-2 and GLPA-3) by a combination of ethanol precipitation,ion-exchange and gel permeation chromatography.All the fractions were analyzed by determining monosaccharide composition,molecular weight distribution.The results show that GLPN-1 was mainly composed of Gal(38.1%) and Ara(33.6%) and presumed to be an arabinogalactan.GLPA-1a,GLPA-1b and GLPA-2 mainly contained Gal,Ara,Rha and GalA.The amount of Gal and Ara acounted for over 50% of total sugar and the amount of Rha was more than 10%.The molar ratios of Rha/GalA in GLPA-1a,GLPA-1b and GLPA-2 were 1:1,1:2 and 1:2.7,respectively,indicating that they belong to type-I rhamnogalacturonan (RG-I)-rich pectin.GLPA-1c was mainly composed of GalA(78%),belonging to homogalacturonan(HG)-rich pectin.GLPA-3 might be a glycoprotein.
NI Xiu-zhenWANG Bing-qingZHI YuanWEI Ning-ningZHANG XuLI Shan-shanTAI Gui-huaZHOU Yi-faZHAO Ji-ming
