Water and nitrogen are primary limiting factors in semiarid grassland ecosystems. Our knowledge is still poor regarding the interactive effects of water and N addition on soil microbial communities, although this information is crucial to reveal the mechanisms of the terrestrial ecosystem response to global changes. We addressed this problem by conducting a field experiment with a 15% surplus of the average rainfall under three levels of N addition（50, 100, and 200 kg N ha–1 yr–1） in two consecutive years in Inner Mongolia, China. Microbial community composition and functional diversity were analyzed based on phospholipid fatty acids（PLFA） and BIOLOG techniques, respectively. The results showed that water addition did not affect the soil microbial community composition, but much more yearly precipitation generally decreased the PLFA concentration, which implied a fast response of soil microbes to changes of water condition. Soil fungi was depressed only by N addition at the high level（200 kg N ha–1 yr–1） and without hydrologic leaching, while Gram-negative bacteria was suppressed probably by plant competition at high level N addition but with hydrologic leaching. The study found unilateral positive/negative interactions between water and N addition in affecting soil microbial community, however, climate condition（precipitation） could be a significant factor in disturbing the interactions. This study highlighted that：（1） The sustained effect of pulsed water addition was minimal on the soil microbial community composition but significant on the microbial community functional diversity and（2） the complex interaction between water and N addition on soil microbial community related to the inter-annual variation of the climate and plant response.
SUN Liang-jieQI Yu-chunDONG Yun-sheHE Ya-tingPENG QinLIU Xin-chaoJIA Jun-qiangGUO Shu-fangCAO Cong-cong
The need is pressing to investigate soil CO2 （carbon dioxide） emissions and soil organic carbon dynamics under water-saving irrigation practices in agricultural systems for exploring the potentials of soil carbon sequestration. A field experiment was conducted to compare the influences of drip irrigation （DI） and flood irrigation （FI） on soil organic carbon dynamics and the spatial and temporal variations in CO2 emissions during the summer maize growing season in the North China Plain using the static closed chamber method. The mean CO2 efflux over the growing season was larger under DI than that under FI. The cumulative CO2 emissions at the field scale were 1959.10 and 1759.12 g/m2 under DI and FI, respectively. The cumulative CO2 emission on plant rows （OR） was larger than that between plant rows （BR） under FI, and the cumulative CO2 emission on the irrigation pipes （OP） was larger than that between irrigation pipes （BP） under DI. The cumulative CO2 emissions of OP, BP and bare area （BA） under DI were larger than those of OR, BR and BA under FI, respectively. Additionally, DI promoted root respiration more effectively than FI did. The average proportion of root respiration contributing to the soil CO2 emissions of OP under DI was larger than that of OR under FI. A general conclusion drawn from this study is that soil CO2 emission was significantly influenced by the soil water content, soil temperature and air temperature under both DI and FI. Larger concentrations of dissolved organic carbon （DOC）, microbial biomass carbon （MBC） and total organic carbon （TOC） were observed under FI than those under DI. The observed high concentrations （DOC, MBC, and TOC） under FI might be resulted from the irrigation-associated soil saturation that in turn inhibited microbial activity and lowered decomposition rate of soil organic matter. However, DI increased the soil organic matter quality （the ratio of MBC to TOC） at the depth of 10-20 cm compared with FI. Our results sugge
We examined the effects of simulated rainfall and increasing N supply of different levels on CO2 pulse emission from typical Inner Mongolian steppe soil using the static opaque chamber technique, respectively in a dry June and a rainy August. The treatments included NH4NO3 additions at rates of 0, 5, 10, and 20 g N/（m2.year） with or without water. Immediately after the experimental simulated rainfall events, the CO2 effluxes in the watering plots without N addition （WCK） increased greatly and reached the maximum value at 2 hr. However, the efflux level reverted to the background level within 48 hr. The cumulative CO2 effluxes in the soil ranged from 5.60 to 6.49 g C/m2 over 48 hr after a single water application, thus showing an increase of approximately 148.64% and 48.36% in the efftuxes during both observation periods. By contrast, the addition of different N levels without water addition did not result in a significant change in soil respiration in the short term. Two-way ANOVA showed that the effects of the interaction between water and N addition were insignificant in short-term soil COz efftuxes in the soil. The cumulative soil CO2 fluxes of different treatments over 48 hr accounted for approximately 5.34% to 6.91% and 2.36% to 2.93% of annual C emission in both experimental periods. These results stress the need for improving the sampling frequency after rainfall in future studies to ensure more accurate evaluation of the grassland C emission contribution.
Yuchun QiXinchao LiuYunshe DongQin PengYating HeLiangjie SunJunqiang JiaCongcong Cao
