It is very important to understand the initial plastic behavior of metals at microscale.In order to research the initial plasticity of body centered cubic metals in micro-/nano-scale,the multiscale simulation method and experimental study were used to study the nanoindentation process of Fe single crystal.The results show that the first abruption of load-displacement curve in nanoindentation of Fe single crystal can be attributed to the first transition from elastic to plastic deformation characterized by the dislocation emission.