In order to investigate the effects of afforestation on soil microbial abundance, microbial biomass carbon and enzyme activity in sandy dunes, 20-year-old Pinus sylvestris var. mongolica Litv. （PSM） and Populus simonii Carri6re （PSC） mature forests were se- lected in Horqin Sandy Land, and mobile dunes was set as a control （CK）. Results show that PSM and PSC plantations can im- prove soil physicochemical properties and significantly increase microbiological activity in mobile dunes. Soil microbial abun- dance, microbial biomass carbon and enzyme activity show an order of PS〉PSM〉CK. Total soil microbial abundance in PSM and PSC was respectively 50.16 and 72.48 times more than that in CK, and the differences were significant among PSM, PSC and CK Soil microbial biomass carbon in PSM and PSC was respectively 23.67 and 33.34 times more than that in CK, and the difference was insignificant between PSM and PSC. Soil enzyme activity, including dehydrogenase （DEH）, peroxidase （PER）, protease （PRO）, urease （URE） and cellobiohydrolase （CEL） in PSM and PSC were respectively 19.00 and 27.54, 4.78 and 9.89, 4.05 and 8.67, 29.93 and 37.46, and 9.66 and 13.42 times of that in CK. R sylvestris and P. simonii can effectively improve soil physico- chemical and microbiological properties in sandy dunes and fix mobile dunes in Horqin Sandy Land. The Cmic：C ratio is an appli- cable indicator to estimate soil stability and soil water availability, and based on an overall consideration of plantation stability and sustainability, R sylvestris is better than R simonii in fixing mobile dunes in sandy land.
Plant root exudates contain various organic and inorganic components that include glucose, citric and oxalic acid. These components affect rhizosphere microbial and microfaunal activities, but the mechanisms are not fully known. Studies concerned from degraded grassland ecosystems with low soil carbon（C） contents are rare, in spite of the global distribution of grasslands in need of restoration. All these have a high potential for carbon sequestration, with a reduced carbon content due to overutilization. An exudate component that rapidly decomposes will increase soil respiration and CO2 emission, while a component that reduces decomposition of native soil carbon can reduce CO2 emission and actually help sequestering carbon in soil. Therefore, to investigate root exudate effects on rhizosphere activity, citric acid, glucose and oxalic acid（0.6 g C/kg dry soil） were added to soils from three biotopes（grassland, fixed dune and mobile dune） located in Naiman, Horqin Sandy Land, Inner Mongolia, China） and subjected to a 24-day incubation experiment together with a control. The soils were also analyzed for general soil properties. The results show that total respiration without exudate addition was highest in grassland soil, intermediate in fixed dune and lowest in mobile dune soil. However, the proportion of native soil carbon mineralized was highest in mobile dune soil, reflecting the low C/N ratio found there. The exudate effects on CO2-C emissions and other variables differed somewhat between biotopes, but total respiration（including that from the added substrates） was significantly increased in all combinations compared with the control, except for oxalic acid addition to mobile dune soil, which reduced CO2-C emissions from native soil carbon. A small but statistically significant increase in pH by the exudate additions in grassland and fixed dune soil was observed, but there was a major decrease from acid additions to mobile dune soil. In contrast, electrical conductivity decreased in grassland
The Horqin Sandy Grassland is one of the most seriously desertified areas in China＇s agro-pastoral ecotone due to its fragile ecology, combined with improper and unsustainable land management. We investigated organic carbon changes in bulk soil （0 to 5 cm）, light fraction of soil organic matter, and soil particle-size fractions induced by land-use and cover type changes. The results indicated that total soil organic carbon （SOC） storage decreased by 121 g/m^2 with the conversion of grassland into farmland for 30 years, and increased by 261 g/m^2 with the conversion of grassland into plantation for 30 years. Total SOC storage decreased by 157 g/m^2 as a result of severe grassland desertification due to long-term continuous livestock grazing, whereas total SOC increased by 111 g/m^2 following the practice of grazing exclusion （16 years） in desertified areas. Changes in land-use and cover type also show great effects on carbon storage in soil physical fractions.
China＇s Horqin Sandy Land,a formerly lush grassland,has experienced extensive desertification that caused considerable carbon（C） losses from the plant-soil system.Natural restoration through grazing exclusion is a widely suggested option to sequester C and to restore degraded land.In a desertified grassland,we investigated the C accumulation in the total and light fractions of the soil organic matter from 2005 to 2013 during natural restoration.To a depth of 20 cm,the light fraction organic carbon（LFOC） storage increased by 221 g C/m2（84%） and the total soil organic carbon（SOC） storage increased by 435 g C/m2（55%）.The light fraction dry matter content represented a small proportion of the total soil mass（ranging from 0.74% in 2005 to 1.39% in 2013）,but the proportion of total SOC storage accounted for by LFOC was remarkable（ranging from 33% to 40%）.The C sequestration averaged 28 g C/（m2·a） for LFOC and 54 g C/（m2·a） for total SOC.The total SOC was strongly and significantly positively linearly related to the light fraction dry matter content and the proportions of fine sand and silt＋clay.The light fraction organic matter played a major role in total SOC sequestration.Our results suggest that grazing exclusion can restore desertified grassland and has a high potential for sequestering SOC in the semiarid Horqin Sandy Land.
YuQiang LIXueYong ZHAOFengXia ZHANGTala AWADAShaoKun WANGHaLin ZHAOTongHui ZHANGYuLin LI
