为了筛选获得高效拮抗建兰炭疽病病原菌胶孢炭疽菌(Colletotrichum gloeosporioides)的菌株,采用平板对峙法,从新疆库姆塔格沙漠土样分离的126株细菌中初筛获得23株拮抗菌株,经平板扩散法复筛得到1株高效拮抗菌株N147-1-3。结合菌落菌体形态观察、16S r DNA序列分析和生理生化试验,鉴定该菌为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。对拮抗菌发酵液上清测定表明,60%的硫酸铵沉淀物具有最高的抑菌活性,经氯仿抽提后萃取相不具有拮抗活性,经高温处理和蛋白酶K消化后抑菌物质的拮抗作用消失,初步分析抑菌物质是蛋白类物质。
Different ground mulching treatments were designed to observe the effects on soil moisture content in a second-generation seed orchard of Pinus elliottii. The results showed that： （1） there were extreme differences in soil moisture content Ⅰ and soil moisture content Ⅲ, but no obvious difference in soil moisture content II was observed; and （2） the high-to-low order of soil moisture content Ⅰ under different ground mulching treatments was white film〉sawdust〉black film〉the control, and the highest value was higher than the lowest one by 26.69%; the high-to-low order of soil moisture content Ⅱ under different ground mulching treatments was white film〉black film〉sawdust〉the control, and the highest value was higher than the lowest one by 20.64%; and the high-to-low order of soil moisture content Ⅲ under different ground mulching treatments was black film〉white film〉sawdust〉the control, and the highest value was higher than the lowest one by 26.61%.
