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吉林省自然科学基金(990544)

作品数:3 被引量:31H指数:3
相关作者:陈霞杨百全江林靳健杨文胜更多>>
相关机构:吉林大学吉林省公安厅通化市卫生学校更多>>
发文基金:吉林省自然科学基金国家自然科学基金更多>>
相关领域:理学医药卫生政治法律生物学更多>>

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硅珠法在纯化污染检材中的应用被引量:6
2003年
目的改进硅珠法在纯化污染DNA样本中的应用方法。方法对硅珠法中的具体步骤进行改进,直接纯化、浓缩Chelex法获得的不纯DNA,采用IdentifilerTM试剂盒进行复合扩增,产物经ABI3100测序仪分型检测。结果纯化后的DNA获得了满意的DNA分型。结论硅珠法能够有效地去除DNA样本中的污染物,直接用于纯化Chelex-100法提取的DNA。
杨百全李晨旭付伟东陈霞李祥顺
关键词:DNA检验
DNA模板诱导针状CdS纳米粒子的形成被引量:20
2000年
Monolayer of octadecylamine(ODA) and salmon sperm DNA or salmon sperm DNA-Cd complex were studied on air-water interface by π-A isotherm. After being co-transferred onto substrates by Langmuir-Blodgett technique, atomic force microscope(AFM) measurements show the DNA molecules are packed into lines in the film, due to the interactions between the ODA and DNA molecules. By exposing the ODA and DNA-Cd complex LB film to H2S, needle-like CdS nanoparticles were formed along the DNA lines as characterized by transmission electron microscope(TEM). Electron diffraction(ED) image indicates that the so prepared needle-like CdS is a new kind of nanostructured materials.
陈霞靳健杨文胜郭中满江林杨百全徐力魏莉李铁津郑大方
关键词:DNA模板剂
DNALB膜的AFM形貌观察被引量:5
2000年
DNA/octadecylamine(ODA) monolayers were transferred onto silicon substrates and the morphologies of the monolayers were investigated by Atomic Force Microscope(AFM). AFM images show that the morphologies of DNA dissolved in pure water are very different from those of DNA dissolved in the NaCl solution. When DNA molecules are dissovled in pure water, they will form ball-like structure in the monolayer. When the DNA molecules are dissolved in NaCl solution, they will form bunch lines. This DNA line offers a valuable template to direct the formation of unique inorganic nanomaterials.
陈霞靳健杨文胜杨百全徐力江林白玉白李铁津陈曦
关键词:LB技术DNA分子AFMLB膜纳米材料
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