The purpose of this study was to overcome the disadvantages of ammonia-nitrogen wastewater pollution and high cost of sodium saponification in rare earth separation process. The study focused on the non-saponification extraction technology with magnesia. The influences of the content and particle size of magnesia, reaction time, reaction temperature, and O/A on cerium extraction rate were also discussed. The results showed that the hydrogen ions of extractant were exchanged by rare earth ions when organic extractant and rare earth solution were mixed with magnesia powder, and then the exchanged hydrogen dissolved magnesia to make the acidity of the system stable. The magnesium ions were not participated in the extraction reaction. Non-saponification extraction process of rare earth had been realized. The cerium extraction rate could reach up to 99% in single stage within the optimal reaction conditions.
[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9(1C~*). [Method] With super japonica rice Songjing 9 as the receptor, transgenic cry1C~* super japonica rice Songjing 9(1C~*) regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Bt-m RNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay(ELISA); the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-m RNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9(1C~*) significantly differed among strains; there was no correlation relationship in Bt-m RNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-m RNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-m RNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9(1C~*), whether the Bt gene expression was high or low, showed good resistance against C. suppressalis.This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C~* super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-m RNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice. [Conclusion] The Bt gene and its expressio
This paper investigates the morphology and crystallization properties of the two crystalline phases of pentacene grown by thermal evaporation on p+-Si substrates at room temperature by the methods of atomic force microscopy and x-ray diffraction. This kind of substrate induces a thin film phase and a triclinic phase which are formed directly onto p+-Si substrates and constitute a layer consisting of faceted grains with a step height between terraces of 15.8 (1=0.1nm) and 14.9, respectively. Above the critical thickness of the thin film phase, lamellar structures are found with an increasing fraction with the increase of the film thickness. When the film thickness is fixed, the fraction of lamellar structures increases with the increase of annealing temperature. These lamellar structures are identified as the second phase with a interplanar distance of 14.9 corresponding to the pentacene triclinic phase. Furthermore, the thin film phase consisting of several micrometre sized uniformly oriented grains at an annealing temperature of less than 80°C and a deposition rate of 0.6 /s is observed.