Supersonic extraction process of glycyrrhizia polysaccharide(GPS) was examined by orthogonal experiments . The optimized supersonic extraction process was obtained and mathematical simulation based on the supersonic extraction was established. The optimized condition for extraction of GPS is as follows: supersonic treatment time is 3 5 h, supersonic treatment temperature is 81 ℃, liquid solid ratio is 10 mL/g, number of times of extraction is 1. Under the optimized condition, the extraction rate of GPS is 5 40%, purity is 39 95% and the relative deviation of repeated experiments is 2 54%.
采用超临界二氧化碳萃取法(SFE CO2 )提取的乌拉尔甘草种子油,用气相色谱质谱(GC MS)联用技术对其挥发性成分进行了分析,首次分离得到52个峰,确定了其中的16种化合物;采用峰面积归一化法计算各成分相对百分含量,所鉴定成分占总馏出峰面积的80. 85%。主要成分邻苯二甲酸二丁酯和β谷甾醇乙酸酯具有较多的生理活性,为进一步开发甘草种子油新药研究提供了依据。
This paper presents a vacuum-cavitated extraction method for glycyrrhizic acid(GA) extraction from supercritical extraction residue.The optimum extraction conditions are as follows:0.3% ammonia solution as the solvent,the ratio of solvent to solid 10∶[KG-*3/5]1(mL/g),vacuum-cavitated time 15 min,and the crude (extract) yeild is 30.3%,in which the content of GA is 6.6%,so the GA extraction yeild is 2.0%.There are three extraction methods:soxhlet,ultrasonic wave,and vacuum-cavitated extraction methods.The results show that the extraction yield of GA by vacuum-cavitated extraction method is 1.6 times that by soxhlet extraction method and 1.2 times that by ultrasonic.At the same time,the vacuum-cavitated extraction method shows many advantages,including lower temperature,less solvent,shorter extraction time,higher extraction yield,and higher GA content in the crude extract.The vacuum-cavitated extraction method is new and highly(efficient),which can be utilized in the pharmaceutical industry.
