以一株分离自黄海海泥(山东日照附近)的海洋放线菌SMA-1为研究对象,利用单菌多次级代谢物(One Strain Many Compounds,OSMAC)方法来增加其代谢物多样性,通过薄层层析和生物活性追踪检测其代谢谱变化,从其中一个活性较强的斑点分离鉴定出活性化合物Naseseazine B,最终建立了一套快速研究海洋放线菌代谢物多样性的培养和分析分离方法。
Natural products(NPs)continue to play a pivotal role in drug discovery programs.The rapid development of synthetic biology has conferred the strategies of NPs production.Synthetic biology is a new engineering discipline that aims to produce desirable products by rationally programming the biological parts and manipulating the pathways.However,there is still a challenge for integrating a heterologous pathway in chassis cells for overproduction purpose due to the limited characterized parts,modules incompatibility,and cell tolerance towards product.Enormous endeavors have been taken for mentioned issues.Herein,in this review,the progresses in naturally discovering novel biological parts and rational design of synthetic biological parts are reviewed,combining with the advanced assembly technologies,pathway engineering,and pathway optimization in global network guidance.The future perspectives are also presented.
