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国家自然科学基金(50830105)

作品数:6 被引量:10H指数:1
相关作者:曹谊林周广东刘伟刘宏伟何斌更多>>
相关机构:上海交通大学医学院附属第九人民医院中国科学院四川大学更多>>
发文基金:国家自然科学基金国家重点基础研究发展计划国家高技术研究发展计划更多>>
相关领域:医药卫生化学工程更多>>

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6 条 记 录,以下是 1-7
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基于软骨细胞的软骨再生核心技术及临床转化
耳廓、鼻、气管和甲状软骨等具有特殊三维立体结构的软骨缺损修复是整复外科领域面临的难题之一,以小耳畸形耳廓缺损为例,目前临床上多采用自体软骨移植或不可降解支架进行修复。前者由于取材于患者本身,会导致机体二次创伤,后者由于缺...
殷宗琦
关键词:耳再造组织工程软骨
聚甘油酸-g-胆固醇胶束的制备及药物释放性能研究被引量:1
2011年
以甘油酸为单体,通过本体缩聚制备了水溶性生物降解高分子聚甘油酸,利用聚甘油酸侧基上的羟基固定生物相容性好的疏水性分子胆固醇,通过亲疏水作用自组装形成胶束.以形成的胶束作为载体负载抗肿瘤药物阿霉素,研究了药物的体外释放行为.将肝癌细胞HepG2与载药胶束共培养研究其体外抗肿瘤效果.研究结果表明,聚甘油酸-g-胆固醇共聚物在水中能够形成高分子胶束,临界胶束浓度为63μg/mL.胶束为球形,粒径分布窄,平均粒径约为120 nm.载药胶束的体外持续释放时间可长达12 h.低浓度时聚甘油酸-g-胆固醇胶束没有细胞毒性;当胶束浓度为1 mg/mL时,共培养的3T3成纤维细胞的活性依然保持90%.体外抗肿瘤效果研究显示载药胶束能够顺利进入细胞,释放药物,抑制肿瘤细胞的增殖,其体外抗肿瘤效果好于自由阿霉素.
黄晓倩何斌宋丽晴杨扬顾忠伟
关键词:生物降解胶束药物释放
Novel PLGGE graft polymeric micelles for doxorubicin delivery被引量:1
2012年
Novel poly{(lactic acid)-co-[(glycolic acid)-alt-(L-glutamic acid)]}-g-monomethyl poly(ethylene glycol) (PLGGE) micelles were prepared and used as carriers for anti-tumor drug delivery. Three PEGylated PLGG copolymers (PLGGE2000, PLGGE1100 and PLGGE500) were characterized by XRD, TG and DSC. The critical micelle concentrations (CMCs) of the amphiphilic copolymers were 1.04, 0.55 and 0.13 μg/mL, respectively. The TEM, AFM and DLS measurements revealed that the micelles were homogeneous spherical nanoparticles with the diameters ranged from 50 to 150 nm when THF was used as solvent in the preparation of the micelles. Interestingly, extended cylindrical micelles were obtained using CHCl 3 as solvent. The micelles could trap doxorubicin (DOX) in the core with the highest drug loading content up to 23.7%. The mean diameter of drug loaded micelles was much bigger than that of blank micelles. The in vitro drug release of the micelles was diffusion-controlled release within the first 36 h and initial burst release was not obvious. However, after 36 h, the release rate in pH 5.0 was faster than that in pH 7.4 due to the degradation. The PLGGE micelles were nontoxic to both NIH 3T3 fibroblasts and HepG2 cells. The in vitro cytotoxicity against HepG2 cells demonstrated that the drug loaded micelles exhibited high inhibition activity to cancer cells. CLSM observation of HepG2 cells showed that DOX released from the micelles could be delivered into cell cytoplasm and cell nuclei. PLGGE micelles are potential promising carriers for anti-tumor drug delivery.
YU ZuXiaoHE BinSHENG MingMingWANG GangGU ZhongWei
关键词:聚合物胶束两亲性共聚物激光共聚焦显微镜聚乙二醇化
COMPARISON OF DRUG DELIVERY PROPERTIES OF PEG-b-PDHPC MICELLES WITH DIFFERENT COMPOSITIONS被引量:1
2012年
An anti-tumor drug doxorubicin was encapsulated in micelles of poly(ethylene glycol)-b-poly(2,2-dihydroxyl- methyl propylene carbonate) (PEG-b-PDHPC) diblock copolymers. The morphololgy of both blank miceiles and drug loaded micelles was characterized by TEM. The in vitro drug release profiles of micelles were investigated, The cytotoxicity of the micelles was evaluated by incubating with Hela tumor cells and 3T3 fibroblasts. The drug loaded micelles were co-cultured with HepG2 cells to evaluate the in vitr9 anti-tumor efficacies. The results showed that the mean sizes of both micelles with different copolymer compositions increased after being loaded with drugs. The drug release rate of PEG45-b-PDHPC34 micelles was faster than that of mPEGt14-b-PDHPC26 micelles. Both of the two block copolymers were non-toxic. The confocal laser scanning microscopy a:ad flow cytometry results showed that both the drug loaded micelles could be internalized efficiently in HepG2 cells. The PEG45-b-PDHPC34 micelles exhibited higher anti-tumor activity comparing to mPEGxla-b-PDHPC26 micelles.
顾忠伟
关键词:DOXORUBICIN
应用比较蛋白质组学技术筛查组织工程化软骨力学性能相关蛋白被引量:1
2013年
目的应用比较蛋白质组学分析方法筛选组织工程化软骨成熟过程中变化显著的细胞外基质蛋白,通过线性相关分析,研究与软骨力学性能相关的蛋白。方法分离人胎儿软骨细胞培养并扩增,接种到聚羟基乙酸/聚乳酸(PGA/PLA)支架材料,体外培养4周后随机分为3组,每组6例,A组继续体外培养6周、B组回植裸鼠体内培养6周、c组回植裸鼠体内培养12周。取材后进行大体观察、湿重及体积测定、组织学及生物力学等相关检测,比较体内外构建软骨与天然软骨形成质量上的差异。同时进行蛋白质组学分析,选择细胞外基质中比值大于2倍的蛋白与相应各组杨氏模量做相关性分析,根据相关系数筛选与软骨力学性能最相关的蛋白。结果3组均能在体内外形成透明软骨样组织,c组和B组软骨外观近似天然成人软骨,组织学显示大量成熟软骨陷窝,而A组形成的软骨组织结构较松散,组织学可见不规则肥大软骨陷窝。c组湿重和生物力学检测分别为(372.5±35.4)mg和(8.68±2.65)MPa,明显高于B组的(346±34.5)mg和(3.25±1.24)MPa(P〈0.01),而A组为(184.4±12.28)mg和(0.7±0.23)MPa。应用蛋白质组学技术检测到细胞外基质中共44个差异蛋白,选择胞外基质中C组和A组之间差异最大的6个蛋白,与相应各组杨氏模量进行线性相关分析,发现核心蛋白聚糖(Decorin)、软骨粘附素(Chondroadhefin)和纤调蛋白(Fibromodulin)与软骨力学性能变化显著相关(P〈0.05)。结论蛋白质组学技术能够提供大量的蛋白质信息,组织构建与比较蛋白质组学技术相结合,有利于进一步分析组织工程化软骨胞外基质蛋白与力学性能的关系。
丁金萍刘宏伟晏丹叶明亮邹汉法刘伟张文杰曹谊林周广东
关键词:软骨蛋白质组学
脂肪干细胞在软骨组织工程中的应用被引量:1
2009年
软骨是最早应用组织工程技术成功构建的组织之一,但由于缺乏合适的软骨构建种子细胞,因此其发展相对落后。随着干细胞研究的兴起,脂肪干细胞(ASC)因其具有分布广泛、可利用细胞量大、取材容易等优点,为ASC作为种子细胞应用于组织工程研究提供了可能;但是ASC构建软骨组织的效果却不如骨髓间充质干细胞(BMSC)理想。因此ASC在软骨组织工程中的应用仍面临着诸多问题与挑战,其中最核心的问题是如何提高ASC成软骨的效率。为此从如何纯化脂肪来源细胞、尽可能保持其中干细胞的生物学特性并优化软骨诱导方案3个方面予以综述,为提高ASC成软骨的效率提供参考。
孙恒赟周广东曹谊林
关键词:脂肪干细胞软骨组织工程种子细胞
Study on the α-cyclodextrin/poly(ethylene glycol) self-assembly supramolecular nanoparticles for drug delivery被引量:5
2010年
This paper reports the synthesis and drug delivery properties of a novel supramolecular nanoparticle.α-Cyclodextrins(α-CD) were threaded on cinnamic acid modified poly(ethylene glycol) to form inclusion complex nanoparticles by supramolecular self-assemble.The anti-tumor drug doxorubicin was loaded in the nanoparticles and released in vitro to study the drug release behavior and the anti-tumor effects.The structure and morphology of the nanoparticles were characterized by nuclear magnetic resonance,X-ray diffraction,ultraviolet absorbance,dynamic laser scattering,scanning electronic microscopy,transmission electron microscopy and atom force microscopy.The distribution of the drug loaded nanoparticles in cells and the anti-tumor effects were studied by confocal laser microscopy.The results demonstrate that the supramolecular nanoparticle is biocompatible and it is a promising carrier for drug delivery systems.
LI Yuan,JI Li,WANG Gang,SONG LiQing,HE Bin,LI Li,NIE Yu,WU Yao & GU ZhongWei National Engineering Research Center for Biomaterials,Sichuan University,Chengdu 610064,China
关键词:SUPRAMOLECULARSELF-ASSEMBLYNANOPARTICLESDRUG
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