目的:通过聚氨酯构建三维(3D)肺癌模型,并用于姜黄素药敏试验。方法:聚氨酯泡沫支架经I型胶原表面修饰后种植A549细胞构建肺癌3D模型,并进行姜黄素药敏试验。扫描电镜观察肺癌A549细胞生长情况。并均与2D培养下进行比较。结果:姜黄素可抑制A549的生长,浓度提高,抑制加强,呈剂量依赖性。3D培养条件下的细胞生长抑制率显著小于2D培养。结论:与2D培养相比,聚氨酯3D肺癌模型可能更好地模拟体内肺癌肿瘤微环境,提高临床前药物研究的预测能力,改善药物临床转化。Objective: To construct a three-dimensional (3D) lung cancer model by polyurethane and use it for curcumin drug sensitivity test. Methods: The 3D model of lung cancer was constructed by transplantation of A549 cells into polyurethane foam scaffolded with collagen type I surface modification, and curcumin susceptibility test was performed. The growth of lung cancer A549 cells was observed by scanning electron microscope. Then the results were compared with 2D culture. Results: Curcumin could inhibit the growth of A549. The concentration increased and the inhibition increased in a dose-dependent manner. The inhibition rate of 3D culture was significantly lower than that of 2D culture. Conclusion: Compared with 2D culture, polyurethane 3D lung cancer model may better simulate the microenvironment of lung cancer in vivo, and improve the predictive ability of preclinical drug studies, and improve the clinical transformation of drugs.
目的探讨初诊为2型糖尿病(T2DM)患者中成人隐匿性自身免疫性糖尿病(LADA)的比例及C肽(CP)在LADA和T2DM鉴别诊断中的价值。方法331例初诊T2DM患者为研究对象,检测胰岛自身抗体,根据胰岛自身抗体结果将患者分为LADA组和T2DM组,比较2组临床资料和生化检测结果,绘制受试者工作特征(ROC)曲线,评价相关指标在鉴别LADA与T2DM的诊断效能。结果初诊为T2DM患者中LADA检出率为9.06%。LADA患者“三多一少”症状和自发性酮症比例、空腹血糖(FPG)、餐后2 h PG和糖化血红蛋白(HbA1c)水平均明显高于T2DM,LADA组病程明显短于T2DM组,起病年龄、BMI、腰围、腰臀比、糖尿病家族史比例和外周血中性粒细胞数(NC)、血清TG、空腹CP(FCP)与餐后2 h CP水平LADA组均明显低于T2DM组,上述差异均有统计学意义(P<0.05)。以0.855μg/L和2.013μg/L分别为FCP与2 h CP鉴别诊断LADA与T2DM的最佳临界值,鉴别诊断LADA与T2DM的准确度明显高于其他指标,差异有统计学意义(P<0.05),其AUC分别为0.866和0.854。结论在T2DM初诊患者中,胰岛自身抗体检测可用于LADA筛查,FCP与2 h CP可作为LADA与T2DM鉴别诊断指标。
