Microbial drugs are antibiotics and other pharmacological substances from the secondary metabolites of microorganisms Screening process includes collection of soil and ocean samples, isolation of microorganisms, fermentation, strain screening, isolation of bioactive components and structure elucidation Producing microorganisms includes streptomyces, rare actinomycetes, bacteria, fungi, and ocean microorganisms New strategy for screening new microbial drugs is the screening of novel anti resistant bacterial agents, antifungal agents, antiviral agents, antitumor substances, immunomodulators and pharmacological active
采用在分离培养基中添加自然来源的抽提液,或加入一些特殊化合物,使其中处于Viable but non-culturable(VBNC)状态的微生物恢复其生长繁殖能力,从而得到分离。实验结果发现甜菜碱、丙酮酸钠、SOD以及过氧化氢酶可使分离到的微生物种类及菌落总数明显增加。还采用固液结合的方法来分离那些在普通平板培养基上不能形成肉眼可见菌落的那些微小菌落的微生物。采用这几种方法从4份土壤样品中共分离得到52株放线菌,103株细菌,17株真菌。对其中的放线菌和真菌进行了生物活性的测定,得到多株具有抗菌活性的微生物,经过多次复筛的平均阳性率为4．325％,略高于用常规方法分离得到的微生物。因此证明有效的分离方法将为今后微生物药物的筛选和药用微生物菌种的保藏提供更丰富的来源。
