目的:本项目计划构建不同雪茄烟气浓度暴露下的小鼠实验模型,探讨雪茄烟气暴露对小鼠口腔菌群的影响。方法:将40只8周龄雄性C57BL/6小鼠随机均分到对照组、雪茄烟气低剂量组、中剂量组以及高剂量组下进行4周饲养,收集小鼠口腔唾液样本,利用16S核糖体RNA (16S ribosomal RNA, 16S rRNA)基因测序技术分析菌群的特征。结果:与对照组相比,不同剂量雪茄干预组小鼠口腔菌群Ace指数以及Shannon指数显著增加(p p p p Objective: This project aimed to develop a mouse model subjected to varying concentrations of cigar smoke to investigate the impact of cigar smoke exposure on the oral microbiome of mice. Method: Forty male C57BL/6 mice, aged 8 weeks, were randomly assigned into four groups: control group, low-dose cigar smoke group, medium-dose group, and high-dose group for a feeding period of four weeks. Oral saliva samples were collected from the mice, and 16S ribosomal RNA (16S rRNA) gene sequencing technology was employed to analyze oral microbiome characteristics. Results: Compared to the control group, the Ace and Shannon index of the oral microbiome in all cigar intervention groups exhibited significant increases (p p p p < 0.05). Conclusion: Cigar smoke exposure and exposure concentrations can affect the microbial composition of the oral microbiome. Additionally, different concentrations of cigars exert specific effects on oral bacteria genera in mice. This study enhances the understanding of how cigar smoke influences oral health and provides critical data for developing effective prevention strategies as well as improving processing and manufacturing methods related to cigars.
