采用摇床培养的方法,以菌丝球的生物量为评价指标,通过单因素试验和正交试验,说明Zn、Fe、Mn、Cu 四种矿质元素对茶树菇液体菌丝生长的影响。结果表明:Zn、Fe、Mn、Cu四种矿质元素对茶树菇液体菌种菌丝生长具有促进作用,其中Zn对茶树菇菌丝生长的影响最明显,其次为Fe、Mn、Cu。这四种矿质元素可以通过单独添加发挥作用,也可以混合添加共同作用,混合添加比单独添加效果更好,促进茶树菇菌丝生长的最优组合是0.25 g/L ZnSO4·7H2O,0.30 g/L FeSO4·7H2O,0.20 g/L MnSO4·H2O,0.15 g/L CuSO4·5H2O。Using the shaking table cultivation method and using the biomass of mycelium balls as the evaluation index, single factor experiments and orthogonal experiments were conducted to illustrate the effects of four mineral elements, Zn, Fe, Mn, and Cu, on the liquid mycelial growth of Agrocybe aegerita. The results showed that four mineral elements, Zn, Fe, Mn, and Cu, had a promoting effect on the mycelial growth of liquid Agrocybe aegerita strains. Among them, Zn had the most significant effect on the mycelial growth of Agrocybe aegerita, followed by Fe, Mn, and Cu. These four mineral elements can exert their effects by adding them alone or by mixing them together. The mixed addition has a better effect than adding them alone. The optimal combination to promote the mycelial growth of Agrocybe aegerita is 0.25 g/L ZnSO4·7H2O, 0.30 g/L FeSO4·7H2O, 0.20 g/L MnSO4·H2O, and 0.15 g/L CuSO4·5H2O.
