Considerable studies on molecular mechanism of brain ischemia reperfusion had been conducted in recent years,including the changes of calcium homeostasis,the gengeration of free radicals and the disorder of mitochondria function,activation of protein kinase,and the expression of gene inflammation and so on.Among them,the disorder of mitochondria function plays an important role in brain ischemia reperfusion Injury.Aiming at the change of cytoplasm,mitochondria calcium homeostasis and disorder of mitochondria function,we discuss the role played by them in brain ischemia reperfusion
Objective To investgate the effects of propofol on cell apoptosis of focal cerebral ischemia-reperfusion injury in the rat model of reversible middle cerebral artery occlusion(MCAO).Methods Male SD rats were used to undergo 2 h MCAO and 2 h reperfusion 2.Brain cell apopotosis and expression of bcl-2 protein was measured by flow cytometry(FCM),expression of caspase-3 protein was observed by immunohistochemical method;brain cell structure was examined under light microscope.Results Propofol could significantly inhibit the apoptosis of brain cell after focal cerebral ischemia-reperfusion.Compared to sham group,caspase-3 protein positive-staining cells were remarkably increased in control group,while propofol group were remarkably decreased.Pretreatment of animals with propofol before reperfusion significantly increased the Fluorescent index of Blc-2.Conclusion The increased expression of Caspase-3 may be an important mechanism for brain cell apoptosis after brain ischemia-reperfusion.Propofol possesses the antiapoptotic effects which may be related to the reduction of the expreesion of caspase-3 and increasing the expression of Bcl-2 protein.
