Quadratic regressive factorial experiment was used to optimize concentrations of 6_BA and NAA added in MS midum for shoot multiplication of miniature rose (Rosa cv.Sonnen Kind).The most amount of shoots was achieved by combining 6-BA 1.93 mg·L -1, NAA 0.33 mg·L -1 into MS midum. The 6-BA and NAA concentrations were in a range of 1.68～2.75 mg·L -1 and 0.07～0.54 mg·L -1, respectively, and each 10 explants (stems with one auxiliary bud) cultured on the midum for 40 days, then 50 shoots occurred. The probability of the event was 95%.
