Aeromonas hydrophila is one of the importantly pathogenic bacteria of aquaculture and causes great loss. In this experiment we characterized the response of Aeromonas hydrophila to heat shock by proteomics and found the significant change on its proteome with the rising of temperature. There were 2 novel proteins in the image at 32 ℃ compared with the image at 25 ℃,7 novel proteins at 37 ℃ compared with 32 ℃ and 1 novel protein at 42 ℃ compared with 37 ℃.These novel proteins were mainly appeared at the alkaline zone. The results can elucidate the molecular mechanism that Aeromonas hydrophila is accord with changes of temperature in some extent. The further study to these proteins will contribute to invent a new temperature-sensitive bacterial vaccine.
