[目的]建立23%溴酰·三氟苯悬浮剂中有效成分的分析方法。[方法]采用高效液相色谱(HPLC)法,使用Eclipse Plus C18柱和二极管阵列检测器(DAD),以乙腈和0.1%磷酸水溶液作流动相,分离23%溴酰·三氟苯悬浮剂中的杂质和助剂等,并测定其主要成分溴氰虫酰胺和三氟苯嘧啶的含量。[结果]采用该分析方法,溴氰虫酰胺和三氟苯嘧啶分离效果良好,2者线性相关系数依次为0.9998和0.9997,标准偏差均为0.04,变异系数分别为0.27%和0.46%,平均回收率分别高达99.87%和99.85%。[结论]该分析方法操作简便,分离效果好,精密度和准确度高,可用于23%溴酰·三氟苯悬浮剂中有效组分的定量分析。
The toxicity of insecticides to S.exigua different in generations and instars was tested by topical application. The results that the toxicity of alpha cypermethrin, methomyl, profenofos, and chlorfluazuron to 4th instar was decreased among second, fourth and sixth generations, and that of alpha cypermythrin, methomyl, profenofos, and chlorfluazuron to third, fourth and fifth instar of sixth generation was also decreased. These gave a viewpoint that the resistance develops very quickly with the generations and instars increasing.
