本论文研究植物乳杆菌(Lactobacillus plantarum dy-1)发酵大麦提取物(LFBE)及其主要活性组分对秀丽隐杆线虫脂肪沉积的影响。在建立秀丽线虫肥胖模型的基础上,设计不同浓度提取物及主要活性组分作用于肥胖模型,通过油红O染色法分析秀丽线虫体内脂肪含量变化。结果表明,与模型组相比,LFBE能显著降低秀丽线虫脂肪沉积量,其中浓度为1600μg/mL时,脂肪含量降低了26%,其模型组体脂含量趋于正常组,而未发酵大麦提取物(RBE)则未见此效果;从LFBE中分离出的酚类化合物,总蛋白质,总糖均具有不同程度的减少脂肪沉积的作用,浓度为16μg/mL的酚类化合物、480μg/mL的蛋白质、480μg/mL的总糖,分别能使秀丽线虫脂肪沉积量下降39%、26%及12%。总之,LFBE能显著抑制秀丽线虫体内脂肪的积累,且其中酚类化合物降低脂肪沉积的效果尤为显著。
[ Objective] In order to study the anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of EGB fermentation extraction biotransformed by Hericium erinaceus. [ Method ] The free radical scavenging ability and reducing capacity of DPPH as well as inhibitory rate of nonenzymatic glycation reaction were measured targets for comparing changes of anti-oxidation and inhibitory effect on nonenzymatic glycation reaction of fermentation lyophilizer and fermentation extraction before and after EGB fermention adsorbed by AB-8 macroporous resin. The EGB fermention was biotransformed by Hericium erinaceus. [ Result ] After adsorbed by AB-8 macroporous resin, the bioactive matters were concentrated and separated. The free radical scavenging rate, reducing capacity and inhibitory rate of nonenzymatic glycation reaction were increased significantly after adsorbed by AB-8 macroporous resin. [ Conclusion] AB-8 macroporous resin could be used for preliminary concentration of EGB fermentation which was biotransformed by Hericium erinaceus.
