[Objective] The study aimed to provide the theoretical basis for the development of Saponins isolated from Sapindus mukurossi in the application of pesticides and lay the foundation for the development and utilization of Sapindus species resources in Yunnan province. [ Method] Saponine from S. mukurossi was extracted by the methods of dipping, feed mixed with pesticide, unlimited feeding, and conducted indoor toxicity determination on Plutella xylostella （Linnaeus）, Sitophilus zeamais motschulsky and Drosophila. [ Result] Saponin had no poisoning activity on maize weevil, while it had certain biological activity on diamondback moth and fruit fly, but it had high activity on fruit fly, when the concentration was 50 mg/ml, mortality was 100% at 72 h, LCso was 4.06 mg/ml at 72 h, LTso was 30.19 h when the concentration was 25.000 mg/ml. [Conclusion] Saponins from S. mukurossi could be develooed and utilized as the material for botanical eesticide.
