应用多克隆抗体 c—erbB—2和单克隆抗体 P^(53)对137例乳腺癌作 ABC 法免疫组化染色,并观察肿瘤间质肥大细胞(MC)反应,进行计数。结果显示 c—erbB—2阳性物主要位于细胞膜,53例乳腺癌阳性反应(39%),其中导管内癌强表达6/7(85.7%);P^(53)阳性物主要位于细胞核,36例乳腺癌阳性反应(26.3%),其中髓样癌强表达11/14(78.6%)。MC 在肿瘤边缘间质的数量明显多于肿瘤中心部间质,髓样癌中可见 MC 穿插于癌细胞之间。本组乳腺癌中 c—erbB—2、P^(53)、淋巴结转移阴性组的 MC 计数高于其阳性组,明显负相关。提示肿瘤间质内 MC 对肿瘤细胞的基因表达可能有一定的抑制作用。
Objective To study the expressions of p27,cyclinE and analysis it s meaning in breast carcinoma.Methods By using tissue chip tech-nique creating80c ases breast carcinoma tissue chip.at the same time using the S-P immunohis-to chemical methods,the expressions of p27and cy-clinE in breast carcinoma tissue chip was studied.Results The positive rates of p27and cyclinE were38.8%and52.5%i n80cases breast carcino-ma respectively.A significant correlation was found be tween the lowexpression of p27,overexpression of cyclinE and metastasis of lymph node in breast carcinoma.A significant correlation was found be-tween the lowe xpression of p27and overexpression of cyclinE in breast carcinoma.Conclusion The expression of p27and cyclinE might be considered to be a reference indicator f or prognosis of breast carcinoma.The lowexpression of p27has an ability to effec t the overexpression of cyclinE in breast carcinoma.It is feasible to utilize ti ssue chip for a rapid,economic and accurate screening of clinical tissue specime ns on a large scale.
