Carbon fiber nanoelectrode(tip diameter ca. 100 nm)-patch clamp was firstly applied to real-time monitoring dopamine release from single living vesicles of single Rat pheochromocytoma(PC12) cells with highly temporal and spatial resolution. It is first demonstrated that there are continual vesicle releases of dopamine at the same site in the active release zone, which play a main role in the exocytosis.
A novel nitrogen oxide(NO) sensor fabricated by modifying the only planar surface of a carbon fiber microdisk electrode(CFMDE) with single-walled carbon nanotubes(SWNTs) and Nafion membrane was reported for the first time.The modification of SWNTs dramatically improves the sensitivity of CFMDEs and the detection limit of NO was 4.3 nmol/L.The sensor was successfully applied to the measurement of NO released from single isolated human umbilical vein endothelial cells.
