AIM:To investigate the roles of H pylori vaccine with chitosan as adjuvant in the immunological therapy of H pylori infection. METHODS:Female BALB/c mice infected H pylori were randomly divided into group A,B, C,D and E,which were treated with phosphate buffered saline （PBS）,H pylori antigen,H pylori- antigen plus chitosan solution,H pylori antigen plus chitosan particles,and H pylori antigen plus cholera toxin （CT）,respectively.The vaccine was orally given once a week for 4 weeks.At the 4th week after the last immunization,these mice were killed and gastric mucosa were embedded in paraffin,then assayed with Giemsa and HE staining.At the same time,quantitative H pylori culture was performed,and enzyme-linked im- munosorbnent assay （ELISA） was used to detect anti-H pylori IgA in saliva and gastric mucosa and anti-H pylori IgG in serum. RESULTS:In the groups with chitosan as adju- vant,the eradication of H pylori was achieved in 58.33% mice,which was in accordance with that in the group with CT as adjuvant （45.45%）,and the colonization density of H pylori in the groups with chitosan as adjuvant was significantly lower than that in the other groups （F=24.166,P<0.05-0.001）.The degrees of acute inflammation in gastric mucosal were significantly lower in mice with adjuvant than those in group A and B （H =21.873,P<0.05-0.001）;the degrees of chronic inflammation in gastric mucosal were signifi- cantly lower in the groups with adjuvant chitosan than those in the other groups （H=20.213,P<0.05-0.001）.The levels of specific anti-H pylori IgA in gastric mucosa （60.18±19.87,63.01±20.92, 61.16±22.25） and saliva （3.28±1.38,2.81±1.56, 3.03±1.52）,and specific anti-H pylori IgG （12.73±3.95,12.06±4.84,11.83±4.31） in sera in the groups with adjuvant were significantly higher than those in group A （saliva:1.19±0.63,gastric mucosa:15.56±6.24,serum:6.56±3.50） and group B （saliva:1.32±0.30,gastric mucosa:27.25±6.56,serum:7.86±4.02） （P<0.05-0.001）. CONCLUSION:H pylori vacc
目的研究壳聚糖体内抗幽门螺杆菌(Hp)作用及其对机体 Th 反应的调节作用。方法建立 BALB/c 小鼠 Hp 感染的动物模型后,随机分为8组:(1)对照组;(2)质子泵抑制剂(PPI)组;(3)羟氨苄青霉素(AM)组;(4)AM+PPI 组;(5)壳聚糖组;(6)壳聚糖+PPI 组;(7)壳聚糖+AM组;(8)壳聚糖+AM+PPI 组。每组12只。按组别分别给予上述药物每日2次灌胃,共2周。停药后4周,处死小鼠,无菌条件下取胃黏膜。采用定量 Hp 培养和病理改良 Giemsa 染色法检测胃黏膜内Hp 感染,用定量 ELISA 法检测胃黏膜内 Th1和 Th2细胞因子含量。结果 8组的 Hp 根除率分别为0、0、41.7%、58.3%、58.3%、66.7%、83.3%、91.7%,其中(3)～(8)组的 Hp 根除率显著高于(1)和(2)组(P<0.05),(8)组的 Hp 根除率还显著高于(3)组(P<0.05)。Hp 定植密度研究发现,各组之间 Hp 定植密度差异有统计学意义(P<0.001),(3)～(8)组显著低于(1)和(2)组(P<0.05);(6)～(8)组显著低于(3)组(P<0.05);(8)组显著低于(4)组(P<0.05)。胃黏膜 IL-2含量,组间差异无统计学意义(P>0.05)。胃黏膜 IFN_γ、IL-12、IL-4和 IL-10含量,在含壳聚糖组显著高于不含壳聚糖组(P<0.05)。结论壳聚糖在体内有抗 Hp 作用,并与 AM 有协同作用。壳聚糖可上调胃黏膜局部Th1和 Th2免疫反应,其对机体免疫调节效应可能参与了抗菌机制。
