目的:研究红景天苷对3T3-L1前脂肪细胞增殖分化及Nrf2/HO-1信号通路和脂肪生成转录因子的影响。方法:按文献介绍的方法诱导3T3-L1前脂肪细胞分化,然后分别用红景天苷(50和100μM)、吡格列酮(100μM)及红景天苷(100μM)与HO-1抑制剂ZnPP(3μM)共同处理3T3-L1细胞7天,分别于2、5、7天后进行胞内脂质含量测定,7天后进行胞内TG含量检测,实时定量PCR检测细胞中PPARγ、C/EBPα、SREBP-1c、aP2、adiponectin mRNA表达, Western blot检测胞浆和胞核中Nrf2及细胞中HO-1蛋白表达。结果:红景天苷(50和100μM)可显著抑制胞内脂质的积聚和TG生成,降低脂肪生成相关转录因子PPARγ、C/EBPα、SREBP-1c、aP2、adiponectin mRNA表达,抑制Nrf2核易位,上调HO-1蛋白表达,且量效关系明显;当与ZnPP联用后,上述作用被明显削弱。结论:红景天苷对3T3-L1前脂肪细胞分化和脂肪生成具有显著的抑制作用,其作用机制可能与其激活Nrf2/HO-1信号通路及抑制PPARγ、C/EBPα、SREBP-1c、aP2、adiponectin等脂肪生成转录因子的表达有关。
Objective To explore the best therapeutic method in the treatment of peripheral facial paralysis. Methods One hundred and twenty cases were randomized into a conventional acupuncture group [Yangbai （阳白 GB 24）, Sibai （四白 ST 2）, Yingxiang （迎香 LI 20）, etc.], a Renying （人迎ST 9） acupuncture group and an operation ＋ acupuncture group [acupuncture at ST 9 as the main acupoint and the stellate ganglion block （SGB）], 40 cases in each one. The treatment was given once a day, 7 treatments made one session. After 3 sessions of treatment, the latency and the amplitude of the direct stimulation evoked potential of the facial nerve （ENoG） were compared before and after treatment in three groups, as well as R2 and R2 values of blink reflex （BR）. The total clinical efficacy was assessed. Results The latency of ENoG was shortened and the amplitude was increased significantly in three groups. After treatment, ENoG latency was lower significantly in the operation ＋ acupuncture group as compared with that in the conventional acupuncture group （P〈0.05）. In the Renying （人迎 ST 9） acupuncture group, the amplitude of ENoG was increased as compared with P〈0.05）. After treatment in three groups, those in the other two groups （all R2 and R2 values were decreased significantly. The differences in R1 and R2 values in the Renying （人迎 ST 9） acupuncture group and the operation ＋ acupuncture group before and after treatment were bigger than those in the conventional acupuncture group （all P〈0.05）, and the difference in R2 value in the operation ＋ acupuncture group was bigger than that in the Renying （人迎 ST 9） acupuncture group （P〈O.05）. The clinical markedly effective and curative rate was 87.5% （35/40） in the operation ＋ acupuncture group, which was superior to 77.5% （32/40） in the Renying （）人迎 ST 9）acupuncture group and higher significantly than 65.0% （26/40） in the conventional acupuncture group （P〈0.05）. Conclusion
