The studies of SCAIF I(an unknown protein) by HPLC and ESIMS was reported in this paper. The SCAIF I is a kind of antiangiogenesis extracted from shark cartilage. The extracted protein was purified by HPLC and then studied by ESIMS. The molecule weight of the SCAIF I protein was confirmed to be 15 601 in the first time. Peptide map of the unknown protein was obtained by HPLC ESIMS with a trypsin digestion and possible partial sequences were deduced with the online CID technique. With partial sequence information the homologous proteins are searched with protein database. The homology of SCAIF I summarized in this work is useful in further studies of this unknown protein.
The technique of coupling capillary liquid chromatography (CLC) with ESIMS was introduced in this article. The homemade reverse phase CLC was coupled with ESIMS to analyze the proteolysis of the BSA and TCS. Good separation efficiency is obtained in the peptide mapping and the partial sequence of peptides was deduced in the CID spectrum. Studies show that CLC-MS has high efficiency on sample separation, and has the merits of sensitivity, accuracy and speed on sample identification. CLC has the lower sample consuming and will be more widely used in the proteomics.
