The pollen grain morphology and leaf epiderm shape and microstructural of Rye（ Secale cereal ）, common wheat and Octoploid Trititrigia were observed with the scanning electron microscope（SEM）. The results revealed that there were great differences between pollen and leaf epiderm microcosmic morphology in three species. Such characteristics of Octoploid tnticalwere between those of common wheat and Rye. The morphology of pollen grain and leaf epiderm shape counld be used as an index for taxonomy for genera and species, and had somewhat scientif- ic references to identify new multiploid species created by chromosome engineering.
[Objective] This study was to investigate the difference in microscopic features of pollen grain and stoma of four triticeae species, as well as the relationship between the sizes of pollen grain and stoma, and the chromosome ploidy. [Method] Comparison of the micro-morphological characteristics of pollen grain and leaf epiderm among diaploid Thinopyrum elongatum, Elytrigia intermedia, hexaploid Triticum aestivum and octoploid Tritielytrigia types were carried out by observation under scanning electronic microscope(SEM). [Result] There were some differences among the four species in the micro-morphology, the size, the surface protuberance, the germ pore of pollen grain and the leaf epidermal stoma, in which diploid Thinopyrum elongatum was obviously different from the other three. Diploid, hexaploid and octoploid species of triticeae had remarkable differences in micro-morphological characteristics of pollen grain and stoma. However, some differences between hexaploid species and octoploid species were not significant. [Conclusion] Some microscopic characteristics of pollen grain and stoma could be used as the evidence to identify diaploid, hexaploid and octoploid spieces whose chromosome ploidy are hugely different.
