Adventitious buds were induced by using tender buds emblic ( Phyllanthus emblica L. ) as explants with MS medium contained different concentration of BA and KT, GA 3,NAA or IAA, of which MS medium combined with 0.5 mg·L -1 of BA and 0.1 mg·L -1 of NAA is suitable for adventitious buds inducement, the rate of differentiation reached more than 90%. MS medium with 0.1 mg·L -1 of BA and 0.1 mg·L -1 of NAA is suitable for the growth of shoots, and 2 3 adventitious buds were regenerated in base. But rooting rate reached only about 30% by using 1/2 MS with 0.25 mg·L -1 of NAA or with 0.25 mg·L -1 of NAA and 0.25 mg·L -1 of IBA.
Callus was induced by embryos from mature seeds of Pinus serotina with MS medium contained 0.2 mg·L -1 of KT and 2.5 mg·L -1 of 2,4 D.After successive subculture and selection,six month old callus were transferred to a liquid medium with 0.2 mg·L -1 of KT and 0 5.0 mg·L -1 of 2,4 D and 200 mg·L -1 of LH for vibration culture,the suspension cultured cell lines were established with favorable dispersivity.A great amount of protoplasts were released from three month old cell suspension cultures,and protoplast yield and protoplast viability of suspension cells on linear phase are the highest under the enzyme combination cellulase R 10 2%+pectinase 0.25%?
