The effects of nitrogen on physiological and biochemical characteristics of potato cultivar Zihuabai under NaCl stress were studied in culture condition.The results show that,under NaCl stress,the contents of proline,chlorophyll and protein and root system vitality first increase and then decrease with the increase of nitrogen level,and reach the top under 4.17 mmol/L NH4NO3 level.Wherein,the contents of chlorophyll,protein and root system vitality are respectively 69.88%,13.07% and 59.29% higher than that of the control under 4.17 mmol/L NH4NO3 level;the activities of superoxide dismutase(SOD)and peroxidase(POD)increase generally under NaCl stress with the increase of nitrogen level,and reach the peaks [111.83 U/g and 25.467 U/(g·min)],which are 37.73% and 35.46% higher than that of control,at 6.25 mmol/L NH4NO3 level.